载体吸附培养黄孢原毛平革菌(Phanerochaete chrysosporium)和云芝(Trametes versicolor)对染料的脱色  

Decolorization of dyes by Phanerochaete chrysosporium and Trametes versicolor growing on adsorptive carriers

作　　者：张昊[1] 张文黎 花清信 苌道松[1] 徐颖[1] 李雪玲[1] 任四伟[1] 陈宣宇[1] 杨清香[1] 李学梅[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]焦作卫生医药学校,河南焦作454000 [3]濮阳油田第二高级中学,河南濮阳457001

出　　处：《环境污染与防治》2012年第6期11-15,22,共6页Environmental Pollution & Control

基　　金：国家自然科学基金资助项目(No.20677014);河南省杰出青年基金资助项目(No.104100510006);河南省教育厅自然科学研究计划项目(No.2011B180033)

摘　　要：比较研究了不同载体吸附培养的黄孢原毛平革菌(Phanerochaete chrysosporium)和云芝(Trametes versicolor)对染料连续脱色的效果。结果表明:(1)白腐真菌黄孢原毛平革菌和云芝分别在含有球形的木屑、玉米芯和花生壳载体的液体环境中振荡培养,菌体以膜状或团状形式大量附着生长在载体表面。(2)连续4轮脱色过程中,不论黄孢原毛平革菌还是云芝,都是木屑为载体的培养液的持续脱色和产酶能力最好,宜选择木屑为载体。其中,木屑为载体的黄孢原毛平革菌培养液经过2轮连续12d脱色后对活性黑RB5的最高脱色率仍能达到97%,在第3轮脱色中对活性红M-3BE的最终脱色率接近96%,并且能产生最高611U/L的锰依赖过氧化物酶(MnP)和1 477U/L的木质素过氧化物酶(LiP)。(3)在实际应用中应该将木屑为载体的黄孢原毛平革菌和云芝培养液都投加到含染料废水处理系统中,强化生物处理效果。Phanerochaete chrysosporiurn and Trarnetes versicolor were cultured on different carriers for continu- ous decolorization, the performance of different Phanerochaete chrysosporiurn and Trarnetes versicolor were re- searched and compared. In this paper, Phanerochaete chrysosporiurn and Trametes versicolor were cultured in liquid containing spherical sawdust, corncob and peanut shells under oscillation conditions. During few days of cultivation, large amount of bacteria was grew and attached on the carrier surface in membranous and lump state. After consecu- tive 4 rounds of decolorization, Phanerochaete chrysosporiurn and Trarnetes versicolor growing on sawdust carrier had significant advantage both in continuous decolorization and producing enzymes, so the optimal adsorptive carrier was sawdust. After consecutive 2 rounds and 12 d of reactive black RB5 decolorization, the Phanerochaete chrysosporiurn culture with sawdust as adsorptive carrier could achieve the decolorization of 97%; after three rounds of dye supple- mented, the cultures also could remove nearly 96% of M-3BE and the maximum production of manganese dependant peroxidase enzyme （MnP） and lignin peroxidase enzyme （LIP） was 611, 1 477 U/L. In the actual application, Phan- erochaete chrysosporiurn and Trametes versicolor culture with sawdust as carriers should be added to dye wastewater treatment system to strengthen the biological treatment effect.

关 键 词：载体 黄孢原毛平革菌 云芝 染料脱色 

分 类 号：X172[环境科学与工程—环境科学]