成骨细胞特异性因子2periostin及相关因子在大鼠全层缺损皮肤创面愈合过程中的表达  

The expressions of periostin and the related factors during healing process of full-thickness cutaneous wound in rat

作　　者：侯俊杰[1] 聂芳菲[1] 李博仑[1] 赵霞[1] 秦泽莲[1] 马勇光[1] 李健宁[1] 

机构地区：[1]北京大学第三医院成形外科

出　　处：《中国危重病急救医学》2012年第6期334-337,共4页Chinese Critical Care Medicine

基　　金：国家自然科学基金

摘　　要：目的观察大鼠皮肤缺损创面愈合过程中成骨细胞特异性[大J子2periostin（PN）与血管生成素-1（Ang-1）、血管内皮生长因子（VEGF）及其受体2[VEGFR-2／胎肝激酶-1（FLK-1）]在创面及创缘周围皮肤的表达及对创面愈合的影响。方法48只成年雄性SD大鼠按随机数字表法分成6组，每组8只。在大鼠背中部脊柱两侧各制作一个2μm×2cm全层皮肤缺损创面，分别于术后1、4、7、10、14、21d创面取材，行病理组纵学检查及免疫组化染色检测创面PN、Ang-1、VEGF、FLK-1的表达；并以正常皮肤的表达量作为对照组。结果创面局部组织中各闵子的表达量在术后均迅速升高。与对照组比较，创面PN的表达量在术后1d即增加了234．4％，之后持续升高，至7d增加了597．9％并达到峰值，其后缓慢下降，21d时增长率为280．9％，仍维持在较高水平（均P〈0．05）；Ang-1的表达量在术后1d增加了128．1％，4d增加了327．5％并达到峰值，然后逐渐下降，14d的增长率仅为80．5％，之后轻度升高（均P〈0．05）；VEGF的表达量在术后7d增加了165．8％并达到峰值，而后下降并伴有小幅波动（均P〈0．0S）；FLK-1术后1d增加了56．1％并维持这一水平，至7d增加了70．1％达峰值（均P〈0．05），10d后缓慢下降（均P〉0．05）。结论大鼠皮肤创面愈合过程中，PN、Ang-1、VEGF及其受体FLK-1蛋白均高表达，但各因子的表达高峰和持续时间有所不同。PN的表达量增幅最高且始终维持较高水平至创面表皮化后；说明PN、Ang-1、VEGF、FLK-1共同参与了大鼠皮肤创面的愈合，PN存创面愈合的整个过程中发挥着重要作用。Objective To obsmwe the expression of osteob/ast-specific factor 2 （periostin, PN）, angiopoietin-1 （Ang-1 ）, vascular endothelial gTowth factor （VEGF） and vascular endothelial growth factor receptor-2 [ VEGFR-2/fetal liver kinase-1 （FLK-1 ）; in wound surface and its peripheral skin, and their effects on wound healing in rats. Methods Forty-eight Sprague-Dawley （SD） rats were randomly divided into six groups, with 8 rats in each group. An area of 2 cm × 2 cm full-thickness skin was excised on both sides of the back of rats. Specimens from wounds were obtained on 1, 4, 7, 10, 14, 21 days after operation, and histological evaluation and immunohistochemieal staining of PN, Ang-1, VEGF and FLK-1 were made to determine their expression levels. Normal skin specimens were obtained as normal controls. Results The expressions of PN, Ang-1, VEGF and FLK-1 were signific, antly increased in wound surface after operation. Compared with the skin of normal controls, the expression of PN in the tissues of wound increased by 234.4% on the 1 st day, and then increased continuously up to 597.9% on the 7th day （reaching the peak ） after operation, followed by a decrease, the increase rate was 280.9% on the 21 st day, and still remained at a high level （ all P〈0.05 ）. The expression of Ang-1 in the tissue of wound increased by 128.1% on the 1st day and 327.5% on the 4th day （ reaching the peak）, and then, it was gradually decreased. The increase rate was only 80.5% on the 14th day and it rose slightly later （ all P〈0.05 ）. The expression of VEGF in the tissues of wound reached the peak （ 165.8% ） on the 7th day. Then it decreased with a slight fluctuation （ all P〈 0.05 ）. The expression of FLK- 1 in the tissues of wound was increased by 56.1% on the 1 st day, and the level remained. It reached the peak by an increase of 70.1% on the 7th day （both P〈0.05）. Then, it was lowered after the lOth day （all P〉0.05）. Conclusions The expressions of PN, Ang-1, VEGF, FLK-1 were obviou

关 键 词：成骨细胞特异性因子 创面愈合 血管生成素-1 血管内皮生长因子 血管内皮生 长因子受体2 

分 类 号：R622[医药卫生—整形外科]