针刺“百会”、“大椎”、“足三里”穴对AD模型大鼠学习记忆能力及海马组织内β-AP蛋白表达水平的影响  

Acupuncture “Baihui hole”,“Dazhui”“Zusanli”Points to Learning and Memory Ability and Levels of Beta-AP Protein Expression in the Hippocampus of AD Model rats

作　　者：张玉瑶[1] 王吉锡[1] 刘洋[1] 张宏南[1] 李霞[1] 姜国华[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《黑龙江医学》2012年第4期267-269,276,共4页Heilongjiang Medical Journal

基　　金：黑龙江省中医药科研项目;编号:ZHY10-W21

摘　　要：目的研究电针对AD模型大鼠学习记忆能力及海马组织内β-AP蛋白表达的影响,为临床应用针灸治疗AD进一步提供理论依据。方法选取健康Wistar雄性大鼠50只,进行行为学测试后,选取符合标准的30只大鼠,随机分为正常组、模型组、电针组。造模方法采用微量注射每侧侧脑室10%链脲霉素10μL建立AD模型,电针组经电针刺激"百会"、"大椎"、"足三里"穴进行治疗。用Morris水迷宫检测大鼠的学习记忆能力;用免疫组化染色法检测海马组织内β-AP阳性细胞表达情况。结果电针组大鼠的学习记忆能力显著提高,逃避潜伏期及游泳路程明显缩短(P<0.01),大鼠在原平台象限游泳时间占整个游泳时间的百分比显著升高,第1次穿过原平台所在位置的时间明显缩短(P<0.01);与正常组比较,模型组海马组织内β-AP蛋白表达水平升高(P<0.01),治疗后电针组与模型组比较,β-AP蛋白表达水平降低(P<0.01)。结论电针治疗后AD大鼠学习记忆能力显著改善,针刺可降低AD大鼠海马组织细胞内β-AP蛋白表达水平,说明针刺对AD大鼠具有一定的治疗作用。Objective To study the effect of learning and memory and the protein expression of β - AP in the hip- pocampus in AD model rats, provide a theoretical basis for the clinical application of acupuncture treatment on AD. Methods 50 healthy male Wistar rats, after do behavioral test, 30 selected rats were randomly divided into normal group, model group and EA group. Modeling method was used microinjection of each side of the lateral ventricle of 10% in streptozotocin 10 μL. To stimulate the ＂Baihui＂, ＂Dazhui＂, ＂Zusanli＂ acupoint in EA group. To detect learning and memory ability using the Morris water maze and detect beta - AP - positive cells ex- pression in the hippocampus with immunohistochemical staining. Results The learning and memory ability in EA group was significantly increased. The escape latency and swim distance was significantly shorter （ P 〈 0. 01 ）. It was significant increase in the original platform swimming time percentage of the in whole swimming time. The first time through the original platform lo- cation was significantly shorter （P 〈 0.01 ）. beta - AP protein expression levels of Hippocampus in roodel group increased than normal group （ P 〈 0. 01 ）. Compared with model group, beta - AP protein levels de- creased after treatment in EA group （P 〈 0. 01 ）. Conclusion After EA treatment, learning and memory ability significantly improved in AD rat. Acupuncture can reduce beta - AP protein levels within the ceils of the AD hip- pocampus of rats. Acupuncture has a therapeutic role in AD rats.

关 键 词：阿尔茨海默病 针刺 Β-淀粉样肽 

分 类 号：R245.9[医药卫生—针灸推拿学]