复性速率液相分子杂交法测定芽孢杆菌DNA杂交度  

Determine the DNA binding degree of bacillus subtilis and bacillus pumilus with denaturalization speed in liquid phasic for molecule hybridizition

作　　者：梁峰[1] 刘秀花[1] 刘茵[1] 

机构地区：[1]商丘师专生物系,河南商丘476000

出　　处：《商丘师专学报》2000年第2期97-99,共3页

基　　金：河南省科技攻关计划资助项目 !99116 0 119

摘　　要：报道采用热变性温度法 (Tm)测定枯草芽孢杆菌和短小芽孢杆菌的DNA中G +Cmol%分别为 42 7mol%和 40 2 6mol% ,与文献报道相一致 ,说明该法可为细菌分类学提供可靠的分子生物学数据 .采用复性速率液相杂交法测定上述两菌DNA -DNA杂交度为 9 6 6 % 。This paper report Bacillus subtilis and Bacillus pumilus DNA G+C mol% are 42.7 mol% and 40.26 mol% adopt denaturalization temperature (Tm)methods.The datum closely consistent to literature,and It clew that these datum can used as a credible datum for bacterial taxonomy.Between two strain the DNA-DNA binding degree is 9.66%,with denaturalization speed in liquid phasic for molecule hybridizition,it showed Bacillus subtilis and Bacillus pumilus are two not consanguineous strains in same genus.

关 键 词：芽孢杆菌 复性速率 DNA 液相分子杂交法 杂交度 

分 类 号：Q939.124[生物学—微生物学]