可溶性虫卵抗原对小鼠骨髓源性树突状细胞表型及Th17细胞分化的影响  

Dendritic cells stimulated by the Schistosoma japonicum soluble egg antigen induce the differentiation of Th17 cell

作　　者：薛冰[1,2] 徐璐[1,2] 周亮 仇镇宁[2] 唐奇[2] 李玉华[1,2] 王祝鸣[2] 朱进[1,2] 冯振卿[1,2] 管晓虹[2] 

机构地区：[1]南京医科大学病理学系,江苏南京210029 [2]南京医科大学卫生部抗体技术重点实验室,江苏南京210029 [3]Department of Pathology,Northwestern University,IL 60208,USA

出　　处：《南京医科大学学报（自然科学版）》2012年第5期616-620,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(30972573);国家“863”项目资助(2006AA02Z415)

摘　　要：目的 :探讨日本血吸虫可溶性虫卵抗原(soluble egg antigen,SEA)致敏小鼠骨髓源性树突状细胞(dendritic cells,DCs)后对其细胞表型及Th17细胞分化的影响。方法 :收集BALB/c小鼠骨髓细胞,应用粒细胞-巨噬细胞集落刺激因子(granulocyte-macrophage colony stimulating factor,GM-CSF)、白介素(interleukin,IL)-4定向诱导小鼠骨髓细胞向DCs分化;流式细胞仪检测SEA刺激培养后DCs表面分子的表达情况;流式细胞术检测DCs分泌IL-6、IL-23的水平,ELISA法检测DCs分泌转化生长因子-β(transforming growth factor-β,TGF-β)的水平;SEA刺激DCs与CD4+T细胞共培养后检测培养上清中IL-6、TGF-β、IL-23和IL-17细胞因子水平。结果:诱导培养出可供实验用的高纯度DCs;SEA刺激DCs能表达较高水平的CD80、CD86、CD40、组织相容性复合体(major histocompatibility complex,MHC)-Ⅱ类分子;SEA刺激DCs产生IL-6和TGF-β明显增多;SEA刺激的共培养细胞上清中IL-17水平增高。结论:SEA能够促进DCs成熟,并诱导CD4+T细胞向Th17细胞分化。Objective：To observe the functional changes of routine bone marrow derived dendritic cells （DCs） loaded with the sol- uble egg antigen （SEA）, and its impact on the differentiation of Th17 cell. nethods：Murine bone marrow cells were induced to differ- entiate into DCs by GM-CSF and IL-4. Flow cytometry was used to measure IL-6 and IL-23 and＇ELISA was used to measure TGF-β concentrations in the supernatant of cultured DCs. CD4T ceils and DCs were co-cultured and then the concentrations of relevant cy- tokines in the culture supernatant were detected. Results：Highly purified DCs wire induced and could be used for further experi- ments. DCs were stimulated with SEA to express high levels of CD40, CDS0, CD86 and MHC-II. Meanwhile, DCs stimulated with SEA produced significantly higher concentrations of IL-6 and TGF-β than the blank control group. Co-cultured cells stimulated with SEA yielded significantly higher concentrations of IL-17 than the blank control group. Conclusion：Stimulation by SEA generates mature DCs,and can induce the differentiation of CD4CF cells towards Thl7 cells.

关 键 词：日本血吸虫 可溶性虫卵抗原 树突状细胞 TH17细胞 

分 类 号：Q254[生物学—细胞生物学]