异丙肾上腺素致兔心肌肥厚模型的在体心脏电生理研究  

The electrophysiological research of the cardiac hypertrophy induced by isoproterenol in rabbits

作　　者：胡河[1] 秦牧[1] 刘韬[1] 孔彬[1] 范阳[1] 熊君[1] 黄从新[1] 黄鹤[1] 

机构地区：[1]武汉大学人民医院心内科,湖北武汉430060

出　　处：《中国心脏起搏与心电生理杂志》2012年第2期149-151,共3页Chinese Journal of Cardiac Pacing and Electrophysiology

摘　　要：目的探讨异丙肾上腺素(Iso)导致兔心肌肥厚在体心脏电生理指标的改变及其与室性心律失常(VAs)的关系。方法 24只兔随机分为Iso组(n=12)和对照组(n=12):Iso组给予异丙肾上腺素(0.3 mg.kg-1.d-1)耳缘静脉注射7天,而对照组注射生理盐水(1 ml.kg-1.d-1)7天。运用单相动作电位记录技术和程控刺激技术,观测在体心室部单相动作电位时程(MAPD)、心室有效不应期(VERP)、自发和诱发的室性心律失常。结果与对照组比较,ISO组MAPD30～MAPD90均延长,而且内外膜MAPD梯度增大,在300,400和500 ms固定频率刺激下VERP明显缩短,VAs发生率升高(P均<0.05)。结论心肌肥厚引起心室部MAPD延长和VERP缩短,VAs发生率增加。Objective To establish a rabbit model with myocardial hypertrophy induced by isoproterenol（Iso） and to investigate the change of cellular electrophysiological characteristics and its relationship with the ventricular arrhythmias（VAs） occured after cardiac hypertrophy.Methods Twenty-four rabbits were randomly divided into Iso group（n=12） and control group（n=12）.Iso group received injection of isoproterenol（0.3 mg·kg-1·d-1） via ear vein for 7 days,while the control group injected with saline（1ml·kg-1·d-1） for 7 days.Employing monophasic action potential recording technique and the programmed electrical stimulation technique,monophasic action potential during（MAPD）,ventricular effective refractory period（VERP）,spontaneous and induced VAs were observed.Results In Iso group,MAPD30-MAPD90 lengthened and VERP shorten significantly.In the Iso group,four of twelve occurred with VAs,but one of twelve occurred with VAs in control group,P0.05.Conclusion The prolongation of MAPD and the shortening of VERP in the cardiac hypertrophy induced by Iso increase the occurrence of VAs.

关 键 词：电生理学 异丙肾上腺素 心肌肥厚 单相动作电位 心室有效不应期 

分 类 号：R331.38[医药卫生—人体生理学] R542.2[医药卫生—基础医学]