大花萱草的花梗组织培养与快速繁殖  

Tissue Culture and Rapid Propagation of Pedicels of Hemerocallis hybrida

作　　者：彭广霖[1] 姜宁[1] 潘仕梅[1] 张志芬[1] 史淑一[1] 

机构地区：[1]中国农业大学烟台研究院,山东烟台264670

出　　处：《安徽农业科学》2012年第17期9203-9205,共3页Journal of Anhui Agricultural Sciences

基　　金：中国农业大学(烟台)校级项目(yt2007.14)

摘　　要：[目的]建立大花萱草的花梗组织培养与快速繁殖方法。[方法]以矮化大花萱草的幼嫩花梗为材料,筛选大花萱草快速繁殖中的愈伤组织诱导及丛生芽发生培养基及生根培养基。[结果]MS+2.0～3.0 mg/L 6-BA+0.2～0.3 mg/L NAA、MS+1.0～2.0 mg/L6-BA+0.1～0.2 mg/L NAA及MS和1/2MS+0.2 mg/L NAA培养基分别是愈伤组织诱导、继代培养和生根培养的理想培养基。[结论]该研究所采用的离体培养及簇生苗生根技术,是快速繁殖大花萱草的有效途径,为大花萱草的工厂化生产提供了技术参考。[ Objective ] This study aimed to establish tissue culture and rapid propagation methods for pedicels of HemerocaUis hybrida. [ Method ] Tender pedicels of dwarf H. hybrida were used as experimental materials to select callus induction, subculture and rooting medium for rapid prop- agation of H. hybrida. [ Result ] MS ＋ 2.0 - 3.0 mg/L of 6-BA ＋ 0.2 - 0.3 mg/L of NAA, MS ＋ 1.0 - 2.0 mg/L of 6-BA ＋ 0.1 - 0.2 mg/L of NAA, MS and 1/2MS ＋ 0.2 mg/L of NAA were the appropriate media for callus induction, subculture and rooting, respectively. [ Conclusion ] The in vitro culture and clustered seedling rooting technology used in this study are effective methods for rapid propagation of H. hybrida, which provide technical reference for the industrialized production of H. hybrida.

关 键 词：大花萱草 花梗 离体培养 快速繁殖 

分 类 号：S188[农业科学—农业基础科学]