HPD-400大孔树脂分离纯化栀子黄色素的工艺研究  

Isolation and Purification of Gardenia Yellow Pigment by Macroporous Resin HPD-600

作　　者：高丽[1] 邓青云[1] 姜益泉[1] 李国元[1] 张昭[1] 

机构地区：[1]孝感学院生命科学技术学院,湖北孝感432000

出　　处：《安徽农业科学》2012年第17期9251-9253,共3页Journal of Anhui Agricultural Sciences

基　　金：湖北省自然科学基金重大项目;鄂财企发[2009]91号第931号

摘　　要：[目的]研究大孔树脂分离纯化栀子黄色素的工艺条件。[方法]以9种大孔树脂为对象,考察其对栀子黄色素的吸附和洗脱性能的影响,筛选出适合吸附和分离黄色素的大孔树脂,并考察温度、酸度等因素对HPD-400吸附能力的影响,优选出分离纯化的最佳工艺条件。[结果]HPD-400对栀子黄色素的吸附能力最强,最佳吸附工艺条件为:温度25℃、pH为3.0、粗提液浓度A440 nm为0.590;最佳洗脱工艺条件为:乙醇浓度70%,温度25℃。[结论]HPD-400树脂分离纯化栀子色素的效果良好,可在工业上应用。[ Objective ] To study the absorption and purification conditions of Gardenia yellow pigment by macroporous resin. [ Method ] The best macroporous resin was screened out from nine resins according to their adsorption and elution performance of Gardenia yellow pigment. The influ- ence of temperature, acidity ,etc. on the absorption and desorption of HPD-400 was investigated to explore its best isolation and purification condi- tions. [ Result] HPD-400 showed the largest adsorption capacity, its best adsorption conditions were 25 ℃ temperature ,pH 3.0 and the concentra- tion of crude extracts A440nm = 0. 590; the best elution conditions were 70% ethanol concentration and 25 ℃ temperature. [ Conclusion] HPD-400 shows the best isolation and purification effects of Gardenia yellow pigment,thus can be used in industrialized production.

关 键 词：栀子黄色素 大孔吸附树脂 吸附 解吸 

分 类 号：S567[农业科学—中草药栽培]