迷迭香水提液对大鼠脑缺血再灌注后SOD和·OH的影响  

Effects of Aqueous Extract of Rosemary on ·OH and SOD in the Rats with Cerebral Ischemia and Reperfusion Injury

作　　者：黄宏妙[1] 李灵[1] 王勤[1] 郭占京[1] 周雪梅[1] 

机构地区：[1]广西中医学院药学院,广西南宁530001

出　　处：《安徽农业科学》2012年第17期9262-9263,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究迷迭香水提液对大鼠脑缺血再灌注损伤后超氧化物歧化酶(SOD)活力和羟自由基(.OH)抑制能力的影响。[方法]取健康SD雌性大鼠50只,采用4-血管阻断(4-VO)方法建立全脑缺血再灌注动物模型,造模前灌胃给药7 d,造模后3 h取材,利用分光光度法检测大鼠肝、脑组织中超氧化物歧化酶(SOD)活力和羟自由基(.OH)抑制能力。[结果]与模型组比较,迷迭香水提液各剂量组均能显著提高大鼠各组织中SOD活力(P<0.01)和.OH抑制能力(P<0.05或P<0.01),并存在一定的量效关系,以高剂量组(50 g/kg)效果最佳。[结论]迷迭香水提液对脑缺血再灌注损伤有保护作用,其机制可能与改善氧化应激状态有关。[ Objective ]To investigate the inhibitory effect of the aqueous extract of rosemary on superoxide dismutase （SOD） and hydroxyl radi- cal （ · OH） activity in the rats with whole cerebral isehemia reperfusion injury. [ Method] 50 SD male rats were randomly divided into five groups as followings normal group, model group, rosemary group （10, 30 and 50 g/kg as low, moderate and high dose respectively）. The model of the whole cerebral ischemia reperfusion injury was established by four-vessel occlusion （4-VO） after the continued intragastric administration of rosemary for seven days. Three hours after the establishment of models, the rats were sacrificed. The activities of SOD and the inhibitory action of · OH in the brain and liver tissues were determined by the colorimetric methods. [Result] Compared with the model groups, the activity of SOD and the inhibitory action of ·OH in the cerebral and hepatic tissues were enhanced remarkably （ P 〈 0.05 or P 〈 0.01 ） in all doses of rose- mary, and presented a positive dose-effect relation. The effect was the best with 50 g/kg rosemary. [ Conclusion] Aqueous extract of rosemary had protective effects on the whole cerebral isehemia reperfusion injury in rats, and the mechanism may be involved in oxidative stress.

关 键 词：迷迭香(Rosmarinus OFFICINALIS L.) 脑缺血再灌注 超氧化物歧化酶(SOD) 羟自由基(·OH) 

分 类 号：S567[农业科学—中草药栽培]