仔猪大肠杆菌·C型产气荚膜梭菌菌液的培养工艺  

Study on Culture Technology of Escherichia coli and Clostridium Perfringens Type C of Piglets

作　　者：刘泽文[1] 袁芳艳[1] 周丹娜[1] 梁望旺[1] 段正赢[1] 杨克礼[1] 徐涤平[1] 田永祥[1] 

机构地区：[1]湖北省农业科学院畜牧兽医研究所,湖北武汉430064

出　　处：《安徽农业科学》2012年第17期9276-9277,9279,共3页Journal of Anhui Agricultural Sciences

基　　金：湖北省科技厅引导项目(2006AA201C25);湖北省科技创新中心项目(2007-620-001-03)

摘　　要：[目的]研究仔猪大肠杆菌、C型产气荚膜梭菌菌液的培养工艺。[方法]分别试验不同的培养时间、培养温度和接种剂量对大肠杆菌纤毛表达量和C型产气荚膜梭菌产生毒素的影响。[结果]大肠杆菌在接种剂量为3%～5%,在36～37℃条件下培养7 h,可产生较高含量的纤毛抗原;当接种剂量为1%～2%,C型产气荚膜梭菌C59-2菌株在35℃条件下培养16～20 h,可产生大量致死性毒素。[结论]该研究为研制具有较好效果的仔猪红痢、黄痢预防疫苗提供了依据。[ Objective] The aim was to study the culture technology of Escherichia coli and clostridium perfringens type C of piglets. [ Method ] Effects of different culture time, culture temperature and inoculation dosage on the cilia expression of E. coli and toxin production of clostridium perfringens type C were studied respectively. [ Result ] The results showed that the content of fimbriae antigen was higher when inoculation dosage of E. coli was 3% - 5%, and cultured at 36 - 37℃ for 7 h. There would produce huge amounts lethal toxin when inoculation dosage of clostridium perfringens type C was 1% -2%, and cultured at 35 ℃ for 16 -20 h. [ Conclusion ] The study provides a basis for development with good effect prevention vaccine of red scour of piglets and white scour of piglets.

关 键 词：大肠杆菌 C型产气荚膜梭菌 培养工艺 抗原含量 仔猪红痢 仔猪黄痢 

分 类 号：S852.612[农业科学—基础兽医学]