Opaque-2突变基因(o2)对玉米组合品质的影响  

Nutritional Quality of the Corn Combinations with Mutant Gene Opaque-2(o2)

作　　者：宋丽雅[1] 陈亮[2] 何聪芬[1] 赵刚[2] 白鹏飞[2] 陈岩[2] 常驰[1] 

机构地区：[1]北京工商大学北京市植物资源研究开发重点实验室,北京100048 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《安徽农业科学》2012年第18期9607-9609,9665,共4页Journal of Anhui Agricultural Sciences

基　　金：北京市自然基金(6112003)资助

摘　　要：[目的]探讨o2突变基因的作用方式,提高玉米品质育种研究水平。[方法]通过对18个普通玉米自交系组配的33个普通玉米组合及相应的导入o2近等基因系(o2-NILs)组配的33个含o2玉米组合品质的比较,分析了导入o2后对玉米组合品质的影响。[结果]o2-NILs组合与普通玉米组合相比,其赖氨酸、蛋白质和油份含量有大幅增加,其余14种必须氨基酸含量也发生了相应的变化,天门冬氨酸、苏氨酸、甘氨酸、异亮氨酸、组氨酸、精氨酸和脯氨酸含量提高;而丝氨酸、谷氨酸、丙氨酸、缬氨酸、亮氨酸、酪氨酸和苯丙氨酸含量降低。相关性分析结果表明,天门冬氨酸、精氨酸和苏氨酸变化与赖氨酸变化程度高度相关(>0.8),蛋白质和油份的变化与赖氨酸变化的相关性分别为0.48和0.38,表现为中度相关。33个o2-NILs组合综合品质分析结果表明,o2组合的CAL58×吉477和CA156×196综合品质优良,产量较高,可能具有较大的开发潜力。[结论]该研究结果为今后利用o2对温带玉米种质进行品质改良,并提高改良效率提供了理论和材料基础。[ Objective ] This study aimed to investigate the action of mutant gene o2 and its effect on nutritional quality of different corn combi- nations. [ Method] A total of 33 normal corn combinations from 18 inbred lines were compared with 33 combinations including gene o2 from the corresponding o2 near-isogenic lines （o2-NILs）, to study the effect of o2 gene introduction on grain quality of corn combinations. [ Re- sult ] The contents of lysine, protein and oil in o2-NILs combinations were greatly more than that of normal corn combinations. Except for ly- sine content, contents of other 14 amino acids changed. Contents of aspartic acid, threonine, glycine, isoleucine, histidine, arginine and pro- line improved; while contents of serine, glutamic acid, alanine, valine, leucine, tyrosine and phenylalanine decreased. Correlation analysis showed that contents of aspartic acid, arginine and threonine had the highest correlation with lysine content. Protein and oil contents had high- er correlation with lysine content （0.48 and 0.38）. Analysis of 33 o2-NILs combinations revealed the o2 combinations CAL58 × Ji477and CA156 × 196 showed high comprehensive quality＇ and high yield with greater development potential. [ Conclusion] This study will provide theoretical and material basis for improving the quality of temperate maize germplasm by introducing o2 gene.

关 键 词：玉米 Opaque-2隐性纯合突变基因(o2) 赖氨酸 蛋白质 

分 类 号：S513[农业科学—作物学]