金槐米槲皮素对人鼻咽癌细胞的干预作用研究  

Intervention Effects of Quercetin from Sophora japonica on the Human Nasopharyngeal Carcinoma Cell

作　　者：顾生玖[1] 刘建楠[1] 姚丽新[2] 杨娜[1] 朱开梅[1] 

机构地区：[1]桂林医学院,广西桂林541004 [2]广西壮族自治区南溪山医院,广西桂林541002

出　　处：《安徽农业科学》2012年第18期9639-9640,9668,共3页Journal of Anhui Agricultural Sciences

基　　金：广西科技攻关项目(科技攻关0815005-1-17;0992003A-25;09321056)资助;桂林市科技攻关项目(科技攻关20110106-5)

摘　　要：[目的]探讨金槐米槲皮素对人鼻咽癌CNE2细胞的干预作用。[方法]通过微波辅助提取法从桂北金槐米中提取槲皮素并纯化,用浓度20、40、60和80μmol/L的槲皮素作用于人鼻咽癌细胞CNE2;分别在24、48和72 h后,采用MTT法初步检测人鼻咽癌细胞被抑制的情况,用流式细胞术分析其细胞周期和凋亡率、光学显微镜观察细胞形态学变化。[结果]从槐米中提取的槲皮素对鼻咽癌细胞有明显的抑制作用,且呈浓度、时间明显的依赖性,药物干预作用的最适浓度为60μmol/L,药物干预后形态学观察的最适宜时间为48 h。细胞周期分析显示,槲皮素能阻滞鼻咽癌细胞于G1期。[结论]从桂北金槐米中提取的槲皮素对人鼻咽癌细胞有较强的干预作用。[ Objective ] To discuss the intervention effects of Sophora japonica qucrcetin on the human nasopharyngeal carcinoma cell CNE2. [ Method] Quercetin was extracted from S. japonica and then purified by microwave-assisted extraction. Quercetin was used to interfere human nasopharyngeal carcinoma cell CNE2 with the concentrations of 20, 40, 60 and 80 μmol/L. After 24, 48 and 72 h, the inhibitory situation of human nasopharyngeal carcinoma cell CNE2 was preliminarily detected by MTT method. Cell cycle and apoptosis rate were analyzed by flow cytometry ; and morphological changes were observed by optical microscope. [ Conclusion ] Quercetin extracted from S. japonica had signifi- cantly inhibitory effects on the human nasopharyngeal carcinoma cell, and showed obvious concentration and time dependent manners. The op- timal concentration of drug intervention was 60μmol/L. After drug intervention, the optimal morphological observation time was 48 h. Cell cycle analysis showed that quercetin was able to block nasopharyngeal carcinoma cell at G1 period. [ Conclusion] Quercetin was extracted from S. japonica had intervention effects on human nasopharyngeal carcinoma cell.

关 键 词：金槐米 槲皮素 人鼻咽癌 

分 类 号：S567[农业科学—中草药栽培]