药用植物灵菊七的染色体核型研究  

Study on Chromosomal Karyotypes of Medicinal Plant Gynura medica

作　　者：范三微[1] 董丽辉[1] 凌庆枝[1] 屠晓玮[1] 林芝[1] 高莉莉[1] 魏兆军[2] 

机构地区：[1]浙江医药高等专科学校,浙江宁波315100 [2]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《安徽农业科学》2012年第18期9644-9645,9650,共3页Journal of Anhui Agricultural Sciences

基　　金：浙江省中医药科技计划项目(2011ZB132);浙江省大学生科技创新计划项目(ZJRCKJCX201007)

摘　　要：[目的]研究药用植物灵菊七(Gynura medica)的细胞染色体特点,并对其染色体核型进行分析。[方法]以灵菊七幼苗茎尖为材料,从上午6:30～10:30,每隔20 min取材一次,采用常规制片技术制片,显微镜观察中期分裂相细胞,对染色体完全分散开的细胞进行染色体计数及核型分析。[结果]在染色体分散良好的102个中期分裂相细胞中,有96.08%含20条染色体,3.92%含40条染色体;同时结果表明上午7:50～9:30取材较佳。[结论]G.medica为二倍体植物,体细胞含有10对染色体,核型公式为2n=2x=20 m,染色体核型属于1A型。该研究结果为国内首次报道。[ Objective ] To study the chromosomal characteristics and karyotypes of G. medica. [ Method ] Stem tip of G. medica seedlings were collected every 20 min from 6：30 to 10：30 am, and all materials were treated and squashed for investigating chromosomal number and karyotypes analysis. [ Result] In total 102 stem tip cells in metaphase, 96.08% of cells are with 20 chromosomes, and 3.92% of cells are with 40 chromosomes. The optimal time for sampling was from 7：50 to 9：30 am. [ Conclusion] G. medica is the diploid plant with 20 chromo- somes. Karyotype analysis gets formula 2n = 2x = 20 m, and all 10 pairs of chromosomes belong to type 1A. The article reported the chromo- somes characteristics of G. medica for the first time in China.

关 键 词：灵菊七 染色体 核型 

分 类 号：S567[农业科学—中草药栽培]