压应力对脂肪细胞活性的影响  

Effect of compressive stress on viability of adipocytes

作　　者：赵德梅[1] 孟庆楠[1] 陈建国[1] 唐林平[1] 谭谦[1] 

机构地区：[1]南京大学医学院附属鼓楼医院整形烧伤科,210008

出　　处：《中华医学美学美容杂志》2012年第3期193-196,共4页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：江苏省卫生厅医学重点人才项目（编号：RC2007009）

摘　　要：目的探讨压应力对脂肪细胞活性的影响，为提高自体脂肪移植的成活率及自体脂肪移植的临床应用提供参考。方法将同等条件获取的脂肪颗粒随机分为5组，分别为对照组（OkPa）、25kPa组、50kPa组、75kPa组、100kPa组。用自制的反馈式气控压应力细胞培养装置分别持续加压（对照组不予加压），于加压后1、2、3、4d，分别做葡萄糖转移实验，同时取加压4d后的脂肪颗粒做四甲基偶氮噻唑蓝（MTT）实验以及组织学HE染色，统计学分析比较各组脂肪颗粒活性的大小。结果脂肪颗粒经不同大小压应力作用后，其转移葡萄糖的量随着压应力的增加而减少（P〈0．01）．并且这种影响具有时间依赖性；MTT实验结果显示脂肪颗粒的吸光度值（A492nm）随压应力增加而减少（P〈0．05），且与葡萄糖转移实验相关（r=0．838，P〈0．01）；组织学切片提示脂肪细胞损伤率随压应力增加而增大（P〈0．01）。结论压应力会损伤脂肪颗粒的活性，建议在自体脂肪移植的临床应用中，在受区分离开阔的空间，以尽量减少压应力对脂肪颗粒活性的损伤。Objective To evaluate the viability of adipocytes after they are subjected to different compressive stress in vitro for improvement of autologous fat graft and its clinical application. Methods Fat grafts were harvested from healthy patients who underwent liposuction for body contouring. Then the fat grafts were randomly divided into 5 experimental groups, including control group without any compressive stress, and continuous compressive stress groups （0. 25. 50, 75, and 100 kPa）, and the glucose transport test was performed at days 1, 2, 3 and 4. An MTT assay was also performed after 4 days with continuous compressive stress in each group with the same experimental protocol for control. Routine histological examination was done in all groups to examine possible structural destruction after compressive stress. Results As compared with the control group, the glucose transport test showed transported glucose concentration decreased with an increase in compressive stress in all of the other four groups （P〈0.01）, and this effect would increase with action time. MTT assay showed absorbance （A492nm ） also decreased with an increase in compressive stress in four days （P〈0.05） . the similar fndings of MTT assay on the viability of fat grafts in all five groups and cor- related with the glucose transport test （r= 0. 838, P〈0.01）. Histology showed significantly distorted and fractured adipocytes and cell injury rate was to raise with an increase in compressive stress. Conclusions Our study demonstrates the harmful effect on the viability of fat grafts with an increase in compressive stress and therefore we should reduce this effect in clinical application of fat grafts.

关 键 词：压应力 脂肪细胞 活性 

分 类 号：R622[医药卫生—整形外科]