活性猪脱细胞真皮基质复合自体刃厚皮片移植修复SD大鼠皮肤缺损  

Healing effect of porcine acellular dermal matrix combined with split-thickness autologous skin composites on full-thickness cutaneous wounds in SD rats

作　　者：林才[1,3] 罗旭[1] 万丽[1] 夏卫东[2] 毛葱[3] 

机构地区：[1]温州医学院附属第一医院烧伤科,325000 [2]温州医学院 [3]华南理工大学材料科学与工程学院

出　　处：《中华医学美学美容杂志》2012年第3期197-199,共3页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：浙江省自然科学基金重点项目资助（编号：Z2080985）;温州市科技计划资助项目（编号：Y20080101）;鹿城区科技计划资助项目（编号：S070109）

摘　　要：目的构建具活性细胞成分的激光微孔化猪脱细胞真皮基质（LPADM），并通过I期复合白体刃厚皮片移植观察其在修复SD大鼠全层皮肤缺损中的效果。方法设LPADM组与无孔PADM组，将同种异体成纤维细胞（fibroblast，FB）种植于LPADM上培养，另设仅用FB培养液培养的FB为对照纰，采用双抗体夹心ABC—ELISA法测定各组FB细胞活性因子自细胞介素-10（IL-10）、白细胞介素-6（II-6）、p1转化生长因子（TGF—β1）、层粘连蛋白（I。N）、血管内皮细胞生长因子（VEGF）的表达。制作SI）大鼠全层皮肤缺损伤口模型，将具活性的LPADM复合自体刃厚皮片I期移植，记录其生长情况，定期活检，并进行组织学观察。结果LPADM组、PADM组、对照组各组各时相点各指标吸光度值比较．差异均无统计学意义（F为0．050～1．763，P〉0．05）。移植的LPADM中可见生长良好的FB。移植术后3周，创面愈合良好，未见明显排斥反应。术后1个月，创面瘢痕轻，复合皮肤可提捏。结论用活性LPADM复合自休刃厚皮片I期移植修复SD大鼠全层皮肤缺损，可有效修复创面，并具有较高的生物安全性。Objective To prepare a new type of micropore porcine acellular dermis matrix with the aid of laser （LPADM）, and to validate the healing effect of the LPADM through the phrase I composite transplanting on the back of the full-thickness skin defects in SD rats. Methods In vitro, the allogeneic fibroblasts were separately cultured with the LPADM （LPADM group） or the non-pore PADM （non-pore LPADM group）, while fibroblasts cultured by pure medium were used as control. After culture of 1 day, 3 days and 5 days, the contents of IL-10, IL-6, TGF-β1, LN, VEGF ex- pressed by fibroblasts were determined by double-antibody sandwich ELISA method. In vivo, the phrase I transplantations of LPADM graft with split thickness autologous skin were carried on the hacks of the full-thickness cutaneous defects of SD rats. The healing condition was observed and ana- lyzed by histological tests. Results The differences of the absorbance value between the LPADM group, PADM group and control group in each day were not statistically significant （F = 0. 050- 1. 763, P〉0.05）. The transplantation of LPADM graft with split-thickness autologous skin graft re- suhed in high rate of surviving without signs of rejection 3 weeks later. After i-month of transplanta- tion, the regenerated skin was well enough to be lifted without any serious scars. Conclusions The phrase ] transplantation of LPADM graft with split thickness autologous skin graft can accelerate the healing process of full-thickness skin wounds with high biological safety.

关 键 词：脱细胞真皮基质 成纤维细胞 复合皮 移植 

分 类 号：R644[医药卫生—外科学]