他克莫司联合罗格列酮对TNF-α诱导的HaCaT细胞LL37及NF-kB表达的影响  

Effects of tacrolimus and rosiglitazone on LL37 and NF-κB expression in TNF-αinduced HaCaT cells

作　　者：张翠[1] 刘彦群[1] 魏志平[1] 文晓婷[1] 

机构地区：[1]徐州医学院附属医院皮肤科,江苏徐州221002

出　　处：《中国麻风皮肤病杂志》2012年第6期393-396,共4页China Journal of Leprosy and Skin Diseases

基　　金：中华医学会皮肤性病学分会-安斯泰来制药(中国)有限公司皮肤病学研究基金

摘　　要：目的:确定他克莫司与罗格列酮单独及联合应用对肿瘤坏死因子-α(TNF-α)诱导的HaCaT细胞LI37及核因子-κB(NF-κB)表达的影响。方法:利用TNF-α诱导体外培养的HaCaT细胞处于炎性状态,在不同浓度的他克莫司与罗格列酮单独及联合作用下,采用RT-PCR法检测LI37的表达情况,采用免疫细胞化学(SABC)法检测NF-κB的表达情况。结果:(1)TNF-α诱导的HaCaT细胞中LI37及NF-κB的表达显著高于对照组(P<0.05)。(2)他克莫司、罗格列酮单独及联合应用均可显著抑制TNF-α诱导的HaCaT细胞LL37及NF-κB的表达(P<0.05);二者联合应用的作用效果均较单独用药组更强(P<0.05)。结论:他克莫司和罗格列酮联合应用能增强其对TNF-α诱导的LL37及NF-κB表达的抑制作用。Objective： To determine the effects of tacrolimus and rosiglitazone on the expression of LL37 and nuclear factor- κB （NF—κB） in TNF - α induced HaCaT cells. Method： HaCaT cells were cultured in vitro, and then stimulated by TNF- α to induce inflammation. After treated with tacrolimus and rosiglitazone in different concentration, the expression of LL37 was measured by reverse transcriptase polymerase chain reaction （RT- PCR）, and the expression of NF- κB by immunocytochemistry. Results： （1） The expression of LL37 and NF- κB was significantly increased in TNF - α - induced HaCaT cells than in cells without TNF - α induced （ P 〈 0.05）. （2） Although tacrolimus and rosightazone, alone or in combination, inhibited the expression of LL37 and NF- κB in TNF -α induced HaCaT cells, the effect was stronger when treated in combination than each alone （ P 〈 0.05 ）. Conclusion： Tacrolimus and rosiglitazone can markedly depress the expression of LL37 and NF- κB in TNF- α induced HaCaT cells. The effects were stronger when combination than each alone.

关 键 词：HACAT细胞 他克莫司 罗格列酮 肿瘤坏死因子-α IR37 核因子-ΚB 

分 类 号：R96[医药卫生—药理学]