模拟微重力条件下成骨生长肽促进MC3T3-E1成骨样细胞的增殖与分化  

Osteogenic Growth Peptide Promotes Differentiation and Proliferation of MC3T3-E1 Osteoblasts under Simulated Microgravity

作　　者：刘俊丽[1] 牛忠英[1] 宋淑军[1] 司少艳[1] 周金莲[1] 赵刚[1] 谭小青[1] 张建中[1] 

机构地区：[1]解放军第306医院病理实验科,北京100101

出　　处：《航天医学与医学工程》2012年第3期168-171,共4页Space Medicine & Medical Engineering

基　　金：总装后勤部课题资助项目

摘　　要：目的研究回转模拟微重力(simulated microgravity,SMG)条件下成骨生长肽(OGP10-14)对MC3T3-E1成骨样细胞增殖与分化的影响。方法利用回转器模拟失重,MTT法检测OGP10-14对回转24 h,48h,72 h MC3T3-E1细胞增殖的效应,并观察培养液中碱性磷酸酶(alkaline phosphatase,ALP)、骨桥素(osteopontin,OPN)活性变化。结果回转模拟微重力条件下,10-8mol/L OGP10-14能够显著促进成骨细胞系MC3T3-E1的增殖(P<0.01)并促进成骨细胞系MC3T3-E1的ALP和OPN活性。结论回转模拟微重力条件下,OGP10-14对成骨细胞系MC3T3-E1的增殖与分化具有促进作用。Objective To study the effect of osteogenic growth peptide （ OGPl0-14 ） on the differentiation and proliferation of osteoblast MC3T3-E1 under the conditions of simulated microgravity （SMG）. Methods Under SMG 24 h, 48 h and 72 h, the effectiveness of the proliferation and differentiation of osteoblasts MC3T3-E1 were examined by MTT. Activity changes of ALP and OPN in the cell culture medium was observed after treated with OGP10-14. Results Under SMG, 10-s mol/L OGP10-14 were able to significantly promote the proliferation of osteoblasts MC3T3-EI（P 〈0.01 ） and enhanced the ALP and OPN activity of osteoblasts MC3T3-E1. Conclusion Under SMG, OGP10-14 facilitates the proliferation and differentiation of osteoblasts MC3T3-E1.

关 键 词：模拟微重力 回转器 成骨细胞 成骨生长肽 增殖 分化 

分 类 号：R854[医药卫生—航空、航天与航海医学]