多次5-ALA介导光动力下存活鼻咽癌细胞的生物学行为分析  

Biological behavior evaluation of nasopharyngeal carcinoma cells that survived from multiple 5-ALA mediated photodynamic therapies

作　　者：胡可锦[1] 唐瑶云[2] 赵素萍[2] 

机构地区：[1]韶关学院医学院临床医学系,广东韶关512026 [2]中南大学附属湘雅医院耳鼻咽喉头颈外科,长沙410008

出　　处：《山东大学耳鼻喉眼学报》2012年第3期33-36,共4页Journal of Otolaryngology and Ophthalmology of Shandong University

摘　　要：目的评价多次5-氨基乙酰丙酸(5-aminolevulinic acid,5-ALA)介导光动力下(5-ALA-PDTs)存活鼻咽癌(NPC)细胞亚株CNE2和5-8F的生物学行为。方法采用半数致死剂量(LD50)对细胞亚株CNE2和5-8F间断进行3次PDT处理,收集存活细胞传代扩增,然后分别评价细胞增殖、黏附、迁移能力和血管生成素1、2(Ang-1,Ang-2)表达的变化。结果多次5-ALA-PDTs后存活的CNE2和5-8F在增殖、粘附、迁移能力上均有下降,Ang-1和Ang-2表达下调(P<0.05)。结论多次5-ALA-PDTs可使存活NPC细胞的恶性生物学行为稳定下降,具有减少淋巴转移的潜在价值。Objective To investigate the biological behavior of nasopbaryngeal carcinoma（NPC） cell CNE2 and 5-8F that survived from multiple 5-aminolevulinic acid （5-ALA） mediated PDTs （5-ALA-PDTs）. Methods CNE2 and 5-8F cells were discontinuously treated with median lethal dosage PDT 3 times, surviving cells were collected for cul- ture, then to evaluate the change of proliferation, migration, adhesion and expressions of Ang-1 and Ang-2 in these cells were evaluated. Results The CNE2 and 5-8F cells that survived from multiple 5-ALA-PDTs showed a lower level of proliferation, migration, adhesion and expressions of Ang-1 and Ang-2 （ P 〈 0.05 ）. Condusion Multiple 5-ALA-PDTs could stably decrease the malignant biological behavior of surviving NPC cells, having potential value of reducing lymphatic metastasis.

关 键 词：光动力学治疗 鼻咽癌 生物学行为 血管生成素 

分 类 号：R766[医药卫生—耳鼻咽喉科]