姜黄素对急性腹膜炎大鼠腹膜Toll样受体2和6表达的影响  

Effects of curcumine on expressions of toll-like receptor 2 and 6 in rat peritoneum during acute peritonitis

作　　者：张秀丽[1,2,3] 孙丽 樊怡[2] 王力宁[2] 马健飞[2] 

机构地区：[1]中国医科大学 [2]中国医科大学第一附属医院肾内科,沈阳1100014 [3]本溪市铁路医院血液透析科

出　　处：《肾脏病与透析肾移植杂志》2012年第3期244-248,共5页Chinese Journal of Nephrology,Dialysis & Transplantation

摘　　要：目的:研究表皮葡萄球菌诱发大鼠急性腹膜炎的过程中,姜黄素对Toll样受体2和6(TLR2,TLR6)表达的影响。方法:采用腹腔注射表皮葡萄球菌制备大鼠急性腹膜炎模型,在制模前24h腹腔注射姜黄素[50mg/(kg·d)],然后分别在造模后3h,6h,12h,24h,48h不同时间点处死大鼠并取材,RT-PCR法检测各组大鼠腹膜组织TLR2和TLR6的表达;分别通过WestenBlot、免疫组织化学技术检测各组核因子κB(NF-κB)的表达;ELISA法检测肿瘤坏死因子α(TNF-α)的表达。结果:腹腔注射表皮葡萄球菌后,腹膜组织出现水肿和间皮细胞形态改变。RT-PCR结果显示模型组腹膜TLR2和TLR6的表达自感染后3～24h出现时间依赖性升高。姜黄素处理组较模型组TLR2、TLR6、NF-κB及TNF-α的表达均明显降低。此外,姜黄素处理组腹膜组织损伤轻于模型组。结论:姜黄素能够有效下调在表皮葡萄球菌诱发的急性腹膜炎早期免疫应答过程中TLR2,TLR6和TNF-α的过表达,其作用机制可能是通过NF-κB通路而发挥作用。Objective：Curcumine has a long history of use in Chinese medicine as a treatment for inflammatory conditions. The purpose of this study was to investigate the preventive effects of curcumin against acute peritonitis induced by Staphylococcus epidermidis in rat and to elucidate possible mechanism of Curcumin action, nethodology：Curcumin [ 50 rag/（ kg. d） ] was intraperitoneally injected to rat for 24h, followed by injection of Staphylococcus epidermidis to induce acute peritonitis. After a survival time of 3,6,12,24 and 48 h, TLR2, TLR6 RT-PCR was performed. The expression of NF-κB was analyzed by Western blot and immunohistochemistry,TNF-ct level in rat peritoneal fluid was detected by ELISA. Results：The histological examination with HE staining showed that the submesothelial zone became thickened with the edematous changes and obvious WBC infiltration in both parietal and viscera peritoneum. Curcumin treatment inhibited theelevation of expression of TLR2 and TLR6 in peritoneum of acute peritonitis rats. Both TLR2-and TLR6-RT-PCR were revealed identical patterns in peritoneum of aeute peritonitis rats. The expressions both TLR2 and TLR6 were progressively enhanced in 3h,6h, 12,24 hours after infection. In Curcumin treatment group, there had a similar tendency for the expression of TLR2 and TLR6 in peritoneum of acute peritonitis rats, whereas the expressions were weakened in 6,12,48 after infection compared with model group. The expression of NF-κB at 24 hours after infection and a statistically was signifieant reduction in rat peritoneum as compared to that in model group. Staphylococcus epidermidis could significantly induce the expression of TNF-c~ in rat peritoneal fluid,which could be partially reversed by Curcumin. Conclusion：Our results suggest that Curcumin could attenuate peritoneum tissue injury by inhibiting the expression both TLR2 and TLR6 and the release of inflammatory cytokine TNF-a. These effects may involve downregulation of NF-κB.

关 键 词：腹膜透析 姜黄素 TOLL样受体 核因子ΚB 

分 类 号：R285.5[医药卫生—中药学]