载紫杉醇脂质体微泡联合低频超声干预低龄兔血管结构重建对肝肾功能的影响  

Effects of vascular reconstruction by Paclitaxel-carrying liposome microbubbles combined with low-frequency ultrasound on the liver and kidney function in young rabbits

作　　者：郭军[1] 余更生[1] 计晓娟[1] 白永虹[1] 杨汝铃[1] 王宇[2] 

机构地区：[1]重庆医科大学附属儿童医院心脏中心,儿童发育疾病研究省部共建教育部重点实验室,儿科学重庆市重点实验室,重庆市儿童发育重大疾病诊治与预防国际科技合作基地,重庆400014 [2]重庆医科大学药学院药物化学教研室,重庆400014

出　　处：《重庆医科大学学报》2012年第6期519-522,共4页Journal of Chongqing Medical University

基　　金：重庆市自然科学基金资助项目(编号:2009BB5267)

摘　　要：目的:评价载紫杉醇脂质体微泡(Paclitaxel-carrying liposome microbubbles,PLM)联合低频超声干预低龄兔血管结构重建对肝肾功能的影响。方法:新西兰大白兔24只,日龄(60±15)d,随机平均分为正常组、手术组及干预组,经球囊扩张术建立髂动脉狭窄模型,术后3周采用PLM联合低频超声对狭窄段血管进行处理,处理后1周观察其髂动脉血管管腔面积及内-中膜面积变化情况,并对肝功能及肾功进行检测。结果:与正常组相比,手术组髂动脉内-中膜增厚,细胞排列紊乱,狭窄率高达(80.22±6.7)%,与正常组相比具有显著差异性(P<0.05);干预组髂动脉部分内膜增厚,中膜细胞排列整齐,狭窄率为(16.17±10.45)%,与手术组及正常组相比均具有显著差异(P<0.05);各组肝功能及肾功能检测比较均未见统计学差异(P>0.05)。结论:PLM联合低频超声对低龄兔血管狭窄具有一定干预作用,同时对其肝功能及肾功能无影响。Objective：To evaluate the effects of vascular reconstruction by Paclitaxel-carrying liposome microbubbles（PLM） combined with low-frequency ultrasound on the liver and kidney function in young rabbits.Methods：Twenty-four young New Zealand rabbits aged（60±15） d were randomly divided into normal group,operation group and intervention group.The iliac artery stenosis model was established by balloon dilatation and the intervention model by PLM combined with low-frequency ultrasound at three weeks after the operation.The changes in the lumen area and tunica media-intima area of the iliac artery were measured at one week after the intervention.The liver and kidney function was tested in all groups.Results：The tunica media-intima area of the iliac artery was magnified in operation group compared with that in normal group（P〈0.05）,with disarranged cells and stenosis rate of（80.22±6.7）%.Part of the intima of the iliac artery was thickened in intervention group than in normal group and operation group（P〈0.05）,with arranged cells in tunica media and stenosis rate of（16.17±10.45）%.There was no difference in the liver and kidney function among all groups（P〈0.05）.Conclusion：PLM combined with low-frequency ultrasound can exert intervention effect on the angiostenosis in young rabbits without influencing the liver and kidney function.

关 键 词：紫杉醇 血管结构重建 新西兰大白兔幼兔 肝肾功能 

分 类 号：R725.4[医药卫生—儿科]