阴离子型共聚物中反应性官能团对蛋白质絮凝沉降的影响  

Effects of Anionic Copolymers Containing Reactive Functional Groups on Flocculation of Protein

作　　者：余江[1] 刘会洲[1] 陈家镛[1] 

机构地区：[1]中国科学院化工冶金研究所分离科学与工程青年实验室,北京100080

出　　处：《化工冶金》2000年第2期139-144,共6页

摘　　要：合成了含不同官能团的两种阴离子型共聚物，并研究了它们在不同ｐＨ及离子强 度条件下对模型蛋白质溶菌酶的絮凝沉降行为．结果表明，阴离子共聚物链节中官能团组 合使得调节溶液ｐＨ和离子强度可以控制溶菌酶的絮凝沉降行为．高分子共聚物中官能因 种类及含量对蛋白质的沉降起重要作用．酰胺基因与磺酸基团的协同作用使得二元共聚物 与溶菌酶形成大的絮凝固块而沉降，酰胺基团、磺酸基团及羧酸基团的协同作用使得三元 共聚物能够在较大的ｐＨ范围以及高离子强度下絮凝沉降溶菌酶．Two kinds of anionic copolymers F1024 and F1111 containing reactive functional groups of amino, sulfonic acid and carboxyl groups were prepared by free-radical polymeriza- tion. Studies on flocculation of lysozyme in aqueous solution by the copolymers were carried out. The type and number of the reactive functional groups in copolymers played important roles in affecting the flocculation of lysozyme. Copolymer F1111 showed effective flocculation function at low ionic strength in solution, albeit no flocculation occurred in deionized water solution. The experimental results revealed that the extent of flocculation by F1024 and F1111 decreased with further increasing the concentration of NaCl in the solution. In addition, floc- culation of lysozyme with F1111 can occur at higher ionic strength in solution than that with F1024. Carboxyl groups can provide more negative charge in alkaline solution, and enhance the interaction between copolymer F1111 and the positive charged lysozyme. The synergistic effects among those reactive functional groups (e.g. F1111) contributed to the formation of large size floes to precipitate, and affected the flocculation behavior of protein in solution at various pH values and ionic strength.

关 键 词：阴离子共聚物 反应性官能团 蛋白质 溶菌酶 絮凝 

分 类 号：TQ316.324[化学工程—高聚物工业]