Lgr5在OVA灌胃致敏的BN大鼠肠道上皮中的表达  

Expression of Lgr5 in Intestinal Epithelium in Ovalbumin-sensitized BN Rats

作　　者：马忠校[1] 蔡飒[2] 苏虹[1] 刘兰英[2] 朱清仙[3] 刘志刚[2] 

机构地区：[1]南昌大学研究生院医学部,2009级南昌330006 [2]深圳大学医学院过敏反应与免疫学研究所,广东深圳518052 [3]南昌大学图书馆,南昌330006

出　　处：《南昌大学学报（医学版）》2012年第3期1-3,10,F0003,共5页Journal of Nanchang University：Medical Sciences

基　　金：国家973计划项目(2009CB522108);国家自然科学基金(81000011);深圳大学基础研究科研项目

摘　　要：目的研究卵清蛋白(OVA)灌胃致敏的BN大鼠其肠道自身干细胞参与修复的情况。方法采用免疫组化观察(leucine-rich repeatcontaining G protein-coupled receptor 5,Lgr5)肠道干细胞标记物和增殖细胞核抗原(PC-NA)的组织定位,观察其增生修复情况。用实时荧光定量PCR检测肠道干细胞标记物Lgr5的表达情况。结果 OVA致敏后的BN大鼠,肠上皮充血、水肿,嗜酸性粒细胞增多。Lgr5主要表达于隐窝基底部,与对照组相比PC-NA和Lgr5表达量都有所增加。结论 OVA致敏后,BN大鼠小肠黏膜细胞中PCNA和Lgr5表达量的增加可能与肠道上皮的损伤修复有关。Objective To investigate the effect of intestinal stem cells on tissue repair in ovalbumin（OVA）-sensitized BN rats.Methods Intestinal stem cells were localized by leucine-rich repeatcontaining G protein-coupled receptor 5（Lgr5） immunohistochemistry and cell proliferation was evaluated by proliferating cell nuclear antigen（PCNA） immunohistochemistry.The expression of Lgr5 was detected by real-time PCR.Results After sensitization with OVA,intestinal epithelium showed congestion,edema and increased eosinophils.Lgr5 was mainly located in crypt base cells.Compared with controls,the expression of PCNA and Lgr5 was significantly increased by sensitization with OVA.Conclusion The increase in PCNA and Lgr5 expression in small intestinal mucosa cells may be involved in intestinal epithelial repair in OVA-sensitized BN rats.

关 键 词：富含亮氨酸的G蛋白偶联受体5 卵清蛋白致敏 肠道干细胞 损伤修复 BN大鼠 

分 类 号：R392.8[医药卫生—免疫学]