健康人和乙肝患者血清蛋白质双向电泳图谱比较  

Comparison of Two-dimensional Gel Electrophoresis Maps of Serum Proteins between Healthy Subjects and Hepatitis B Patients

作　　者：吴志鹏[1] 魏碧蓉[2] 谢志勇[3] 林丽彬[1] 

机构地区：[1]莆田学院医学院基础医学部,福建莆田351100 [2]莆田学院医学院护理系,福建莆田351100 [3]莆田学院附属医院检验科,福建莆田351100

出　　处：《南昌大学学报（医学版）》2012年第3期14-16,共3页Journal of Nanchang University：Medical Sciences

基　　金：莆田学院科研启动基金(2006Q014)

摘　　要：目的比较健康人和乙肝患者血清中蛋白质双向电泳图谱的差异。方法通过双向电泳技术对健康人和乙肝患者血清中蛋白质进行双向电泳分离,并对这2种血清蛋白质双向电泳图谱进行对比。结果从健康人和乙肝患者血清中共得到8个显著的差异点。其中在乙肝患者血清中表达量上调有5个点,表达量下调有1个点,乙肝患者血清中新出现2个点。结论健康人和乙肝患者血清蛋白质双向电泳图谱有一定的差异,这些差异点可为进一步筛选乙肝患者血清中具有医学价值的蛋白质提供新的途径。Objective To compare the two-dimensional gel electrophoresis（2-DE） maps of serum proteins between healthy subjects and hepatitis B patients.Methods Serum proteins were separated by 2-DE and the constructed 2-DE maps were compared between healthy subjects and hepatitis B patients.Results Eight different spots were found through comparing the 2-DE maps between healthy subjects and hepatitis B patient.Compared with healthy subjects,5 up-regulated and 1 down-regulated spots were detected in serum proteins from hepatitis B patients.Moreover,2 new spots were found only in serum samples from hepatitis B patients.Conclusion There were some differences in the 2-DE maps of serum proteins between healthy subjects and hepatitis B patients.These different spots may be a promising new way to screen significative proteins in serum samples in hepatitis B patients.

关 键 词：健康人血清 乙肝患者血清 双向电泳图谱 差异点 

分 类 号：R592.1[医药卫生—老年医学] R512.62[医药卫生—内科学]