西瓜枯萎病拮抗细菌HD-5菌株的筛选及鉴定  

The Screening and Identification of Antagonistic Bacterium Strain HD-5 against Watermelon Fusarium Wilt

作　　者：雷白时[1] 王笑颖[2] 郭晓军[1] 姜军坡[1] 朱宝成[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071001 [2]河北农业大学农学院,河北保定071001

出　　处：《湖北农业科学》2012年第11期2236-2238,共3页Hubei Agricultural Sciences

基　　金：河北省自然科学基金项目(303188);河北省教育厅科研基金项目(2002141)

摘　　要：筛选对西瓜枯萎病病原菌西瓜专化型尖孢镰刀菌(Fusarium oxysporum Schl.f.sp.niveum)具有拮抗作用的芽孢细菌。通过对土样中分离的产芽孢细菌进行初筛和复筛,得到一株具有较强抑菌活性的拮抗芽孢细菌HD-5菌株,并对该菌株进行了形态特征观察、生理生化鉴定和16 S rDNA全序列分析。结合HD-5菌株的形态特征、生理生化特性和16 S rDNA全序列分析结果综合考察,判定该菌株为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。In order to obtain antagonistic bacteria against Fusarium oxysporum Schl.f.sp.niveum,84 strains were isolated and screened with the improved agar plate diffusion method using the soil form different districts.The antagonistic activity of strains against F.oxysporum Schl.f.sp.niveum was tested via secondary screening.As a results,strain HD-5 showing the strongest antagonistic activity against F.oxysporum Schl.f.sp.niveum was obtained.Thus the morphology characteristics,physiological and biochemical properties and 16 S rDNA sequence of this strain were studied to determine the species.The similarity of the 16 S rDNA sequences between strain HD-5 and Bacillus subtilis were up to 99.78%.Finally,the strain HD-5 was identified as Bacillus amyloliquefaciens.

关 键 词：西瓜枯萎病 拮抗细菌 解淀粉芽孢杆菌(Bacillus amyloliquefaciens) 筛选 鉴定 

分 类 号：S476[农业科学—农业昆虫与害虫防治]