GnRH在广西摩拉杂交水牛乳腺中的免疫组化定位及其受体基因表达的研究  

Immuno-histochemical Localization of GnRH and Its Receptor Gene Expression in the Mammary Gland of Guangxi Hybrid Murrah Buffalo

作　　者：王秋华[1] 陶春爱[1] 李瑞明[1] 谢莹雪[1] 秦津[1] 凌泽继[1] 宋小白[1] 

机构地区：[1]广西大学动物科学技术学院,南宁530005

出　　处：《湖北农业科学》2012年第11期2277-2280,共4页Hubei Agricultural Sciences

基　　金：广西自然科学基金项目(0640015;桂科自0832043;0991042);广西壮族自治区教育厅科研项目(200709LX075);广西教育厅科学规划课题项目(C类2006C3号);广西大型仪器协作公用网资金项目

摘　　要：采用免疫组化及RT-PCR技术检测广西摩拉杂交水牛乳腺组织中的促性腺激素释放激素(GnRH)及其受体。结果表明,15份正常乳腺组织中有13份呈GnRH免疫阳性反应,免疫阳性产物主要集中在腺泡细胞的胞质和胞膜,胞核无特异性染色;同时扩增的目的条带大小为1 008 bp,与促性腺激素释放激素受体(GnRHR)基因片段大小一致。The gonadotropic releasing hormone（GnRH） and its receptor（GnRH-R） in the mammary gland of Guangxi hybrid Murrah Buffalo were detected by immuno-histochemical and reverse transcription PCR（RT-PCR）.The results showed that in 15 pieces of normal mammary gland tissue,there were 13 samples observed positive immunoreaction.The immunoreactive productions were mainly distributed in the cytoplasm and membrane of acinar cells;and it was negative in nucelus.The amplified target fragment was about 1 008 bp which was conformance to GnRH-R gene fragment.

关 键 词：促性腺激素释放激素(GnRH) 促性腺激素释放激素受体(GnRH-R) 乳腺 免疫组织化学 广西摩拉杂交水牛 基因表达 

分 类 号：S858.315.3[农业科学—临床兽医学]