红阳猕猴桃快速繁殖体系的建立  

Study on Fast Plant Regeneration of Actinidia chinensis

作　　者：朱学栋[1] 刘奕清[2,3,4] 赵荣隆 袁海英 吴林[1] 

机构地区：[1]西南大学园艺园林学院,重庆400716 [2]重庆文理学院花卉研究所,重庆402160 [3]重庆市特色植物种苗工程技术研究中心,重庆402160 [4]重庆高校园林花卉工程研究中心,重庆402160 [5]重庆市巴南区林业局,重庆401320

出　　处：《湖北农业科学》2012年第11期2369-2371,共3页Hubei Agricultural Sciences

基　　金：重庆市森林工程科技支撑重大专项(CSTC;2009AB1122);重庆市科技创新能力建设项目(CSTC;2010CB1012);特色植物种苗工程重庆高校创新团队建设项目(201025)

摘　　要：以红阳猕猴桃(Actinidia chinensis cv.Hongyang)茎段作为外植体进行愈伤组织诱导、不定芽分化和生根培养,建立高效的猕猴桃快速繁殖途径。结果表明,茎段极易产生愈伤组织,在MS+0.8 mg/L ZT+0.1 mg/L NAA培养基中,愈伤组织诱导所需时间短、出愈率高,不定芽的出芽率高、生长健壮;以1/2 MS为基本培养基,添加0.5 mg/L IBA对不定芽生根较为有利,生根率达到93.33%。A stable and efficient in vitro rapid propagation system of Actinidia chinensis cv.Hongyang was established using the stem segments as explants for callus induction,proliferation of adventitious buds and rooting.The results indicated that callus formation of stem segments of A.chinensis was easy.When callus induction and differentiation medium was MS＋0.8 mg/L ZT＋0.1 mg/L NAA,it was with short induction time,high callus induction rate and adventitious buds sprouting rate;and the growth was well.1/2 MS＋0.5 mg/L IBA was good for rooting with a rooting rate of 93.33%.

关 键 词：红阳猕猴桃(Actinidia CHINENSIS cv.Hongyang) 愈伤组织 不定芽 快速繁殖 

分 类 号：S663.4[农业科学—果树学] S339.41[农业科学—园艺学]