羊流产嗜衣原体病和弓形虫病IHA与ELISA诊断方法的比较  

Comparison of IHA and ELISA in Diagnosis of Chlamydophila abortus and Toxoplasma Infections in Goats

作　　者：罗意[1] 周碧君[1,2] 王开功[1,2] 文明[1,2] 程振涛[1,2] 张元鑫 张华 龙冲冲[1] 江楠[1] 

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州省动物疫病研究室,贵州贵阳550025 [3]贵州省动物疾控中心,贵州贵阳550001

出　　处：《动物医学进展》2012年第6期49-51,共3页Progress In Veterinary Medicine

基　　金：贵州省农业科技攻关项目(黔科合NY字[2011-3105];黔科合NY字[2011]3061);贵州省重大科技专项计划项目(黔科合NZ字[2011]3005号);贵州省动物疫病与兽医公共卫生重点实验室培育(黔科合Z字[2011]4008号)

摘　　要：通过比较研究羊流产嗜衣原体与弓形虫抗体的间接血凝试验(IHA)与酶联免疫吸附试验(ELISA)检测结果,选择适宜贵州省山羊流产血清学调查的检测方法。通过IHA和ELISA两种方法对贵州省195份山羊血清进行羊流产嗜衣原体与弓形虫抗体的检测,统计并分析两种疫病的阳性符合率、阴性符合率和总符合率。结果表明,羊流产嗜衣原体与弓形虫IHA与ELISA的总符合率分别为77.95%和78.97%,阳性符合率均仅为50%,阴性符合率为80.45%和79.27%。说明在检测两种疫病血清抗体方面,IHA比ELISA更适合在贵州省基层推广。In order to select suitable detection methods for the serological investigation of goat abortion in Guizhou province, IHA and ELISA were used to detect Chlamydophila abortus and Toxoplasma antibodies from 195 seral samples. The positive coincidence rate, negative coincidence rate and the total coincidence rate of two diseases were analysed. The results shows that IHA and ELISA for total coincidence rate of Chlamydophila abortus and Toxoplasma were 77. 95% and 78. 97% respectively. The both positive rates were only 50% and the negative rates were 80.45% and 79.27% respectively. Compared to ELISA, IHA is more suitable in detecting Chlamydophila abortus and Toxollasma serum antibodies in goats of Guzhou province.

关 键 词：山羊 流产嗜衣原体病 弓形虫病 诊断 

分 类 号：S852.67[农业科学—基础兽医学] S855.91[农业科学—兽医学]