不同早熟凝集染色体方法对^(60)Co γ射线照射的剂量-曲线影响的研究  

Effects of Different Premature Chromosome Condensation Method on Dose-curve of ^(60)Co γ-Ray

作　　者：郭义曹[1] 杨浩贤[1] 杨宇华[1] 李禧娜[1] 黄伟旭[1] 郑巧玲[1] 

机构地区：[1]广东省职业病防治院,广东广州510300

出　　处：《中国辐射卫生》2012年第2期134-136,共3页Chinese Journal of Radiological Health

基　　金：广东省医学科学技术研究基金资助项目(A2008060)

摘　　要：目的研究早熟凝集染色体(PCC)方法学的常规法和改进后的方法对60Coγ射线剂量-效应曲线影响,为事故应急选择快速和准确的生物剂量估算方法。方法取3名23～28岁的男性肘静脉血,在60Coγ射线照射(01、.0、5.0、10.0、15.02、0.0 Gy,吸收剂量率0.635 Gy/min)的条件下,以常规PCC培养方法为对比;比较2种方法(常规法和改进法)的50 h和60 h的2个培养时间所产生剂量-效应曲线的关系;以另一实验室照射10.0 Gy(吸收剂量率0.670 Gy/min)所产生的PCC环结果,用上述制作的剂量-曲线估算剂量作验证。结果①常规50 h培养方法15.0、20.0 Gy点所获PCC细胞数不能满足统计学要求,常规方法60 h、改进法50和60 h的3个培养方法收获的细胞数均可满足统计学要求,故后3个方法均取作剂量曲线;②后3个培养方法的PCC环与受照剂量的相关系数其系数间相当接近(均0.996以上、均P<0.01),直线回归方程成立,相关指数亦相当接近,剂量-效应拟合均为线性模式,回归系数间差异无统计学意义(P<0.05),回归直线的形态基本重叠;③验证:本研究的3条剂量-曲线估算受照10.0 Gy的结果误差均≤8%,均符合生物学实验一般允许的15%。结论本研究的3个方法制作的3条曲线均可用于大剂量电离辐射损伤的生物剂量估算,但以改进法的50 h培养速度快,可作为事故应急首选的方法。Objective To study the effect of traditional method and improved method of the premature chromosome condensation（PCC） on the dose -eftect curve of 60Coγ ray, for choosing the rapid and accurate biological dose estimating method for the accident emergency. Methods Collected 3 healthy male cubits venous blood （23 to 28 years old） ,and irradia- ted by 0,1.0,5.0,10.0,15.0,20.0 Gy ~）Co ~ ray （absorbed dose rate：0. 635 Gy/min）. Observed the relation of dose - effect curve in the 2 incubation time（50 hours and 60 hours） of the traditional method and improved method. Used the dose - effect curve to verify the esposure of 10.0 Gy（ absorbed dose rate ：0. 670 Gy/min）. Results （~）In the traditional method of 50 - hour culture,the PCC cell count in 15.0 Gy and 20.0 Gy was of no statistical significance. But there were statistical signifi- cance in the traditional method of 60 - hours culture and improved method（50 - hour culture and 60 - hour culture）. Used the last 3 culture methods to make dose curve. （~）In the above 3 culture methods,the related coefficient between PCC ring and ex- posure dose was quite close （ all of more than 0.996,P 〈 0.01 ） , the linear regression equation was established and the correla- tion index was quite close, the dose -effect curve was fitting the linear model. But there were no statistical significance among regression coefficient（ P 〉 0.05 ） ,the morphology of regression straight lines almost overlap. （~）Used the above 3 dose - effect curves to estimate the irradiation results（ 10.0 Gy） ,the error was less than or equal to 8% ,all of them were within the allowa- ble range of the biological experiment（ 15% ）. Conclusion The 3 dose - effect curves of the above 3 culture methods can ap- ply to biological dose estimating of large doses of ionizing radiation damage. Especially the improved method of 50 - hour cul- ture,it is much faster to estimate and it should be regarded as the first choice in accident emergency. [ Key words] Prema

关 键 词：早熟凝集染色体(PCC) 方法学比较 剂量-曲线估算 事故应急 

分 类 号：R144.1[医药卫生—公共卫生与预防医学]