早期显性梅毒患者血清IL-2、IL-10和TNF-α检测及其临床意义  

Detection and its clinical significance of IL-2,IL-10 and TNF-α in the sera of patients with early symptomatic syphilis

作　　者：胡晓燕[1] 聂芳[1] 

机构地区：[1]白银市第一人民医院检验科,甘肃白银730900

出　　处：《检验医学》2012年第6期451-453,共3页Laboratory Medicine

摘　　要：目的探讨白细胞介素-2(IL-2)、白细胞介素-10(IL-10)和肿瘤坏死因子-α(TNF-α)在早期显性梅毒发病机制中的作用。方法采用双抗体夹心酶联免疫吸附试验(DAbS-ELISA)检测53例早期显性梅毒患者(其中一期梅毒25例、二期梅毒28例)和65名正常人血清IL-2、IL-10和TNF-α水平。结果早期显性梅毒患者血清IL-2、IL-10和TNF-α水平明显高于正常对照组(P<0.01);一期梅毒患者血清IL-2和TNF-α水平明显高于二期梅毒患者(P<0.01),血清IL-10水平则低于二期梅毒患者(P<0.01);早期显性梅毒患者中IL-2与IL-10呈负相关(r=-0.760,P=0.000),与TNF-α呈正相关(r=0.633,P=0.000),而IL-10与INF-α无明显相关性(r=-0.063,P=0.575)。结论 IL-2、IL-10和TNF-α均参与了梅毒的发病,IL-10表达上调可能是二期梅毒发生辅助性T(Th)1/Th2细胞免疫应答失衡的主要原因之一。Objective To investigate the role of interleukin-2 （IL-2）, interleukin-10 （IL-10） and tumor necrosis factor-alpha（TNF-α） in the pathogenesis of early symptomatic syphilis. Methods Double-antibody sandwich enzyme-linked immunosorbent assay（DAbS-ELISA） was used to detect the serum levels of IL-2,IL-10 and TNF-α in 53 patients with early symptomatic syphilis （25 cases with primary syphilis and 28 cases with secondary syphilis ） and 65 healthy subjects. Results Serum levels of IL-2, IL-10 and TNF-α in early symptomatic syphilis group were significantly higher than those in control group （ P 〈 0.01 ）. Serum levels of IL-2 and TNF-α in primary syphilis group were significantly higher than those in secondary syphilis group （P 〈 0.01 ）. Serum level of IL-10 in secondary syphilis group was significantly higher than that in primary syphilis group （P 〈 0.01 ）. IL-2 and IL-10 were negatively correlated in early symptomatic syphilis group （ r = - 0. 760, P = 0. 000）. IL-2 and TNF-α were positively correlated （ r = 0. 633, P = 0. 000） , and IL-10 and α were not correlated（r = -0. 063,P = 0. 575 ）. Conclusions IL-2, IL-10 and TNF-α are involved in the pathogenesis of syphilis. Up-expression of IL-10 may contribute to the helper T cell （Th）1/Th2 immune response imbalance during the development of early symptomatic syphilis.

关 键 词：白细胞介素-2 白细胞介素-10 肿瘤坏死因子-Α 梅毒 

分 类 号：R446.62[医药卫生—诊断学]