芒柄花黄素对超氧阴离子及羟自由基清除作用的研究  

Scavenging effects of formononetin on superoxide anion free radical and hydroxy free radical

作　　者：叶雨[1] 陈健[2] 

机构地区：[1]广西医科大学第一附属医院西院急诊科,广西南宁530021 [2]桂林医学院基础医学院生理教研室,广西桂林541004

出　　处：《四川生理科学杂志》2012年第2期53-55,共3页Sichuan Journal of Physiological Sciences

基　　金：广西医学科学实验中心开放基金(编号:KFJJ2011-34)

摘　　要：目的:探讨芒柄花黄素体外对氧自由基的抑制和清除作用。方法:采用分光光度法检测芒柄花黄素对超氧阴离子自由基(O2-)和羟自由基(.OH)的清除及抑制作用。结果:芒柄花黄素终浓度为10、20、40μg.ml-1时,在体外对O2-的清除率分别为42.19%,56.64%及65.43%,对O2-生成的抑制率分别为31.06%,44.72%及63.35%;而对.OH的清除率分别为34.98%,41.06%及61.60%。结论:芒柄花黄素对O2-具有明显的抑制及清除作用,其对.OH也有明显的清除作用。Objective： To study the scavenging effects of formononetin on superoxide anion free radieal（O2- ） and hydroxy free radical（OH ）. Methods： Speetrophotometry （wave length 322 nm and 510 nm） was used to observe the effects of Formononetin on O2- and ·OH, respectively. Results： The level of O2- was reduced by 42.19%, 56.64%and 65.43% in the presence of Formononetin （10, 20, 40μg ml^-1） in vitro, the generation of O2- was inhibited by 31.06%, 44.72% and 63.35%, respectively, and the level of ·OH was reduced by 34.98%, 41.06% and 61.60%, respectively. Conclusion. Formononetin can greatly scavenge O2-, inhibit the production of O2-, and scavenge ·OH.

关 键 词：芒柄花黄素 超氧阴离子自由基 羟自由基 

分 类 号：R285.5[医药卫生—中药学]