鲍活体倍性检测技术的研究  

Detection technology research of ploidy on live abalone

作　　者：林美金 

机构地区：[1]福建省宁德市蕉城区水产技术推广站,福建宁德352100

出　　处：《福建水产》2012年第3期225-230,共6页Journal of Fujian Fisheries

基　　金：国家农业科技成果转化项目<鲍多倍体育种技术应用示范>(项目编号:2011GB2C400010)

摘　　要：在生物有机体中,一个细胞核中所含的DNA总量对于某种类有机体是一定的。利用流式细胞仪(FCM)测量细胞中的DNA含量,可以用来鉴别有机体的倍性。本研究分别取杂交鲍[即皱纹盘鲍(Haliotis discus hannai)×盘鲍(Haliotis.discus discus)]的一小块触手、足肌,设计不同的振荡时间、不同的DAPI染色时间、不同的取样部位,制备成不同的细胞悬液检查样品;用PA-Ⅱ型倍体分析仪测定其倍性,以探讨其对倍性检测效果的影响。试验结果表明:振荡的最适时间为1.5～2.0 min,染色的最适时间为4.0～5.0 min;取材部位的不同对检测结果无显著影响。The total DNA amount in a cell nucleus varies with different species, the species DNA ploidy can be determined according to the analysis of the DNA content of the cell by Flow Cytometry. In the present stud- y, the DNA ploidy determination for hybrid abalone （ 9 Haliotis discus hannai x 5 Haliotis discus discus） was studied. The cells suspension was provided from small tentacles of hybrid abalone, then the staining was conducted by Destination Access Point Identifier （ DAPI）, and finally, DNA ploidy determination was fin- ished by PA- 11 ploidy analyzer. The results showed that the optimal time for oscillation was 1.5 -2.0 mi- nutes, the optimal staining time was 4.0 -5.0 minutes.

关 键 词：倍性检测 流式细胞仪 DAPI 

分 类 号：Q-33[生物学]