寒凝血瘀对结肠癌肺转移模型小鼠肺组织VEGF、MMP-2表达的影响  

Effect of Blood Stasis Due to Cold on the Expression of Lung Tissue VEGF and MMP-2 in Mice with Metastatic Lung Lesions from Colon Cancer

作　　者：周华妙[1] 郭勇[1] 

机构地区：[1]浙江省中医院,杭州310006

出　　处：《浙江中西医结合杂志》2012年第6期429-432,共4页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的:观察寒凝血瘀状态下Balb/c小鼠C26人工血行肺转移组织血管内皮生长因子(VEGF)、基质金属蛋白酶-2(MMP-2)表达。方法:将小鼠随机分为空白对照组、寒凝血瘀组、单纯荷瘤组、复合造模组,分别进行造模,通过免疫组化、Western Blot方法检测肺转移灶中VEGF、MMP-2的表达。结果:复合造模组小鼠基质金属蛋白酶-2(MMP-2)表达高于单纯荷瘤组,差异有统计学意义(P<0.05),复合造模组小鼠血管内皮生长因子(VEGF)表达较单纯荷瘤组小鼠有增高趋势,差异无统计学意义(P>0.05)。结论:寒凝血瘀状态可能通过促使结肠癌小鼠肿瘤组织VEGF和MMP-2表达的上调,促使小鼠C26结肠癌血行肺转移,促进疾病进展。Objective：To investigate the role of blood stasis caused by cold in the expression of vascular endothelial growth factor（VEGF）and matrix metalloproteinase 2（MMP-2） in lung tissues of Balb/c mice with metastatic lung cancer. Methods： Twenty-four Balb/c mice were equally randomized into 4 groups： normal control group, blood stasis group, colon cancer group, and blood stasis-cancer group. Models were established in other 3 groups except the normal controls. The expression of VEGF was detected with immunohistochemistry and MMP-2 with Western blotting. Results： The expression of MMP-2 in the blood stasis-eancer group was significantly higher than that in the cancer group（P〈0.05）. The expression of VEGF in the blood stasis-cancer group was higher than that in the cancer group without a significant difference（P〉0.05）. Conclusion： The state of blood stasis caused by cold may prompt the hematogenons metastasis of colon cancer to the lung by upregulating the levels of VEGF and MMP-2 in tumor tissues.

关 键 词：小鼠 结肠癌 血行转移 寒凝血瘀 基质金属蛋白酶-2 血管内皮生长因子 

分 类 号：R273[医药卫生—中西医结合] R735.35[医药卫生—中医肿瘤科]