月季“黄和平”体细胞胚诱导的初步研究  

Prelimitary Study on Somatic Embryo Induction in Rosa chinensis

作　　者：尤扬[1] 金典生[2] 

机构地区：[1]河南科技学院园艺园林学院,河南新乡453003 [2]河南机电高等专科学校,河南新乡453000

出　　处：《湖北农业科学》2012年第10期2128-2131,共4页Hubei Agricultural Sciences

基　　金：河南科技学院重点科研基金资助项目(050122)

摘　　要：以月季(Rosa chinensis)"黄和平"的幼嫩叶片为外植体,研究了不同植物生长调节剂对其愈伤组织和体细胞胚诱导的影响。结果表明,在愈伤组织诱导试验中,MS+3 mg/L 2,4-D+0.5 mg/L 6-BA效果最好,诱导率达94.8%;在体细胞胚诱导试验中,其最适诱导培养基为MS+1.2 mg/L TDZ,暗培养8 d后转入光下培养,体细胞胚诱导率最高,达33.3%,暗培养超过8 d,外植体易形成愈伤组织而不利于体细胞胚的诱导。Using tender leaves of Rosa chinensis cv.’Yellow Peace’ as explants,the effects of different growth regulator on tissue culture and somatic embryogenesis induction were studied.The results showed that MS＋3 mg/L 2,4-D＋0.5 mg/L 6-BA was the best for callus induction,with an induction rate of 94.8%.In the somatic embryogenesis induction test,dark induction for 8 d in MS＋1.2 mg/L TDZ before light culture,the regeneration rate was the highest,with an induction rate of 33.3%.If dark culture time was longer than 8 d,callus would easily formed while the regeneration frequency could be reduced.

关 键 词：月季(Rosa chinensis) 叶片 愈伤组织 体细胞胚 

分 类 号：Q949.751.8[生物学—植物学] Q813.12