化痰通络法对急性脑梗死大鼠溶栓后血脑屏障通透性及海马内皮细胞超微结构的影响  

Effect of Huatan Tongluo on the permeability of blood-brain barrier and ultrastructure of hippocampus endothelial cells after thrombolysis in acute cerebral infarction

作　　者：周震[1] 宋宛珊[2] 王占奎[1] 毛蕾[2] 马妍[2] 张玉莲[1] 

机构地区：[1]300150天津,天津中医药大学第二附属医院 [2]天津中医药大学

出　　处：《中华行为医学与脑科学杂志》2012年第6期508-511,共4页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目（81072796）;天津市应用基础及前沿技术研究计划项目（10JCZDJC20500）

摘　　要：目的观察化痰通络法对急性脑梗死大鼠重组组织型纤溶酶原激活物（rt—PA）溶栓后神经功能缺损评分、血脑屏障通透性及海马内皮细胞超微结构的影响，为化痰通络法防治急性脑梗死溶栓后出血转化提供实验依据。方法选择健康雄性SD大鼠120只分为6组，即假手术组、模型组、rt—PA组、低剂量中药组、中剂量中药组、高剂量中药组（每组又分为6h、24h、3d、7d四个时相），采用自身栓子法制备大脑中动脉栓塞（MCAO）模型大鼠，rt—PA组及中药组分别给予尾静脉注入rt—PA及联合3种不同剂量化痰通络中药干预。根据不同观察时相对大鼠进行神经功能缺损评分（Bedersong评分），采用依文思蓝染色（EB染色）检测血脑屏障（BBB）通透性，并通过电镜对大脑海马缺血区内皮细胞超微结构进行观察。结果1．Bedersong评分：与模型组相比，n．PA组、低剂量中药组、中剂量中药组、高剂量中药组神经功能缺损评分明显降低（P〈0．01）；与rt-PA组相比，以rt—PA联合化痰通络法中剂量组、高剂量组神经功能缺损评分降低最为明显（P〈0．05）。2．EB染色：与模型组相比，rt—PA组、低剂量中药组、中剂量中药组、高剂量中药组EB含量均明显减低（P〈0．01）；与rt—PA组[6h：（236．72±79．79）ng／g；24h：（229．72±22．18）ng/g；3d：（163．34±24．45）ng／g；7d：（133．21±25．09）ng／g]相比，以化痰通络法联合rt—PA中剂量组[6h：（121．63±33．27）ng／g；24h：（153．78±60．67）ng／g；3d：（76．05±27．11）ng／g；7d：（72．97±25．81）ng／g]、高剂量组[6h评分（123．11±23.65）ng/g；24h评分（138．36±52．36）ng/g；3d评分（83．00±32．00）ng/g；7d评分（75．06±13．97）ng／g]EB含量降低最为明显（P〈0．01）。3．电镜观察：与模型组相比，治疗组均起到保护内皮细胞的作用，其中以rt�Objective To observe the effects of Huatan Tongluo on the Bederson＇s scores , the permeability of blood-brain barrier and uhrastructure of hippocampus endothelial cells after thrombolysis in acute cerebral infarction. To provide experimental evidence for the prevention of bleeding after thrombolysis in acute cerebral infarction with Huatan Tonghm. Methods 120 SD male rats were randomly divided into sham group,model group, rt-PA group, Huatan Tongluo combined rt-PA low-dose group, Huatan Tongluo combined rt-PA medium-dose group and Huatan Tongluo combined rt-PA high-dose group（ each group was divided in 6h, 24h, 3d, 7d four-phase）. MCAO rats were prepared by self-embolus method, rt-PA group and Huatan Tongluo combined rt-PA groups were given rt-PA through tail vein injection and three doses of Huatan Tongluo to treat. To observe the Bederson＇s scores in rat on four-phase. To detect the permeability of blood-brain barrier（BBB） by the Evans Blue dye （EB dye） and watch the uhrastructure of hippocampus endothelial cells in ischemic region by electron microscopy. Results 1. Bederson＇s scores：Compared with model group, rt-PA group, Huatan Tongluo combined rt-PA low-dose group, Huatan Tongluo combined rt-PA medium-dose group and Huatan Tongluo combined rt-PA high-dose group significantly lower（P〈 0.01 ）; Compared with rt-PA group, Huatan Tongluo combined rt-PA medium-dose groups and high- dose groups was significantly lower （P 〈 0.05 ）. 2. EB dye： Compared with model group, rt-PA group, Huatan Tongluo combined rt-PA low-dose group, Huatan Tongluo combined rt-PA medium-dose group and Huatan Tongluo combined rt-PA high-dose group （P 〈 0.01 ） ; Compared with n-PA group （ 6h ： （ 236.72 ± 79.79 ） ng/g; 24h ： （229.72 ±22.18） ng/g;3d ： （ 163.34 ±24.45） ng/g;7d： （ 133.21 ±25.09） ng/g）, Huatan Tongluo combined rt-PA medium-dose groups （6h： （ 121.63 ±33.27） ng/g;24h： （ 153.78 ±60.67） ng/g;3d： （76.05 ± 27.11 ） ng/g; 7 d ： （ 72.97 ± 2

关 键 词：化痰通络法 脑梗死 溶栓 血脑屏障 内皮细胞 形态学 

分 类 号：R285.5[医药卫生—中药学]