金腰带对脊髓损伤大鼠运动行为学和皮质蛋白激酶B表达的影响  

Effects of Jinyaodai on neurological behavior and Akt expression in the cortex of rats following truamatic spinal cord injury

作　　者：赵波[1] 汤海玲[2] 蔡学光[1] 但齐琴[3] 王廷华[3] 刘佳[4] 

机构地区：[1]650000昆明,云南省第二人民医院放射科 [2]650000昆明,云南省第二人民医院门诊部 [3]四川大学华西医院转化神经科学中心 [4]昆明医科大学神经科学研究所

出　　处：《中华行为医学与脑科学杂志》2012年第6期521-523,共3页Chinese Journal of Behavioral Medicine and Brain Science

摘　　要：目的探讨金腰带治疗对脊髓挫伤大鼠运动功能恢复的影响，并探讨与蛋白激酶B（Akt）表达的关系。方法成年SD大鼠随机分为假手术组，脊髓顿挫伤组、脊髓顿挫伤给予金腰带治疗组（每天1次，连续2周），每组13只动物，其中5只用于神经行为学评价和形态学检测，8只用于RT—PCR。用重力打击法制备脊髓顿挫伤模型。术后每天用金腰带（50mg／kg）对治疗组大鼠进行灌胃。用运动行为学BBB评分评价大鼠后肢运动功能变化；用RT—PCR技术检测各组皮质运动区Akt基因表达变化；用Neun抗体染色计数运动皮质神经元数量变化，并进行统计学分析。结果与假手术组比较，术后1月脊髓损伤大鼠后肢运动功能已明显受损，而金腰带治疗明显促进大鼠神经运动功能恢复（7．8±1．3；14．1±1．4，P〈0．05）；同时明显上调皮质内运动区Akt基因表达水平（0．53±0．05，0．68±0．07，P〈0．05），并增加运动区皮质神经元数量（11±2，15±1；P〈0．05）。结论金腰带治疗可能通过增加皮质内运动区Akt表达，增加备用神经元数量来有效促进脊髓挫伤大鼠运动功能恢复。Objective To explore the effects of Jinyaodai on neurological behavior and Akt expression in the cortex of rats following spinal cord contusion injury. Methods Rats were randomly divided into control group, spinal cord contusion group and Jinyaodai group. The weight-drop device was employed to prepare the spinal cord injury（SCI） model. Jinyaodai was administrated every day by using a stomach tube. Rats were performed the BBB assessment, and the detection of Akt expression and count of Neun positive neurons in cortex following SCI. Results Compared with control group,deficit of motor function in hindlimbs was seen at 3 dpo following cord contusion, and partial functional recovery could be seen from 7 dpo to 1 m. Treatment of Jinyaodai greatly increased the BBB scores （ 14.1 ±1.4 ） more than SCI group （7.8 ± 1.3 ） at 1 month （P 〈 0.05 ） ; Simultaneously, compared with SCI rats,treatment of Jinyaodai significantly increased the expression of Akt（0.53 ±0. 05,0.68 ± 0.07, P 〈 0.05） and the number of neurons （11 ± 2, 15 ± 1; P 〈 0. 05） in the lesion-induced cortex of rats. Conclusion Jinyaodai may play an essential roles in functional recovery after spinal cord injury,in which the underlying mechanism may be involved in the expression of Akt in cortex.

关 键 词：金腰带 脊髓顿挫伤 运动功能 蛋白激酶B 

分 类 号：R651.2[医药卫生—外科学]