水稻籽粒灌浆速率的发育遗传机制研究  

Developmental Genetic Mechanism Research on Grain-filling Rate in Rice

作　　者：贾小丽[1,2] 叶江华[1] 苗利国[2] 林红梅[2] 林文雄[2,3] 

机构地区：[1]武夷学院,福建武夷山354300 [2]福建农林大学农业生态研究所,福建福州350002 [3]教育部生物农药与化学生物学重点实验室,福建福州350002

出　　处：《热带作物学报》2012年第4期622-626,共5页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(No.30871494);教育部博士点基金项目(No.200803890006);福建省自然科学基金项目(No.2012J0s064)

摘　　要：以小穗小粒型水稻Milyang46和大穗大粒型FJCD建立的包含130个家系的E。重组自交系为研究材料，分析福建省武夷山和莆田环境下灌浆期5个阶段灌浆速率的性状表现，并结合已构建的遗传图谱进行QTL动态定位及环境互作研究。QTL分析共检测到10个加性QTL，位于1、2、4、5、8、10号染色体上．对表型变异贡献率为4．52％．17．39％。其中，qGR-5—5、qGR-4—2、qGR-10-1分别在灌浆第2、4、5阶段存在显著的环境互作效应，体现了一因多效。qGR-2—1加性效应可解释表型变异的15．79％。qGR-4—2加性可解释表型变异的17.39％．环境互作效应可解释表型变异贡献率的10．29％。本研究揭示了灌浆速率相关QTL的动态表达．有助于了解籽粒灌浆和产量相关性状的遗传基础。Quantitative trait loci（QTL）dynamic mapping and genexenvironment interaction were used to study grain-filling rate for five stages of grain filling from a RIL including 130 lines deriving from Milyang 46 and FJCD. This study was carried in Wuyishan and Putian, Fujian Province. Ten additive QTL were detected in two environments by QTL mapping analysis and located in chromosome 1, 2, 4, 5, 8, 10. The contribution rates for phenotype variance were from 4.52% to 17.39%. Among them, qGR-5-5, qGR-4-2, qGR-10-1 existed significant additive ~environment interaction effects at 2, 4, 5 stage of grain filling respectively, manifesting ＂pleiotropic effects＂. Additive effect of qGR-2-1 accounted for 15.79% of the phenotypie variation. Additive effect of qGR-4- 2 accounted for 17.39% of the phenotypic variation, and its additivexenvironment interaction effect accounted for 10.29% of the phenotypic variation. This study revealed dynamical expression of QTL associated with grain-filling rate, and contributed to understand the relationship between grain filling and yield-related traits at gene level.

关 键 词：QTL定位 水稻 重组自交系 灌浆速率 环境互作 

分 类 号：Q75[生物学—分子生物学] Q348