穿刺巴斯德芽菌PPh01菌株鉴定及其对根结线虫的寄生性测定  

Identification of Strain PPh01 and Its Parasitism Test to Root-knot Nematode

作　　者：张晓黎[1,2] 商桑[1,3] 朱军[1] 孙前光[1] 贺乐[1] 鲍时翔[1] 黄惠琴[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海南海口571101 [2]海南大学环境与植物保护学院,海南海口570228 [3]海南大学园艺园林学院,海南海口570228

出　　处：《热带作物学报》2012年第4期695-698,共4页Chinese Journal of Tropical Crops

基　　金：973计划前期研究专项(No.2010CB134506);948项目(No.2011-G25);国家自然科学基金项目(No.31170062);海南省自然科学基金(No.311080);海口市重点科技计划(2011-0099);中央级公益性科研院所基金(No.ITBB11-0302;No.ITBB11-0306;No.1630012011004)

摘　　要：从海南定安胡椒根部根结线虫体上分离到一株细菌PPh01,其形态特征与穿刺巴斯德芽菌相似。16SrDNA序列分析结果表明:该菌株与穿刺巴斯德芽菌的同源性达到99.3%,在发育树上聚为同一分支,亲缘关系最近,鉴定该菌株为穿刺巴斯德芽菌(Pasteuria penetrans)。寄生性试验结果表明:该菌株对南方根结线虫、爪哇根结线虫、花生根结线虫的2龄幼虫均具有寄生性和侵染能力。Strain PPh01, isolated from female root-knot nematode of pepper roots at Ding＇an, Hainan Province, was similar to Pasteuria penetrans by morphological characteristics. At the same time, the strain showed the closest 16S rRNA gene sequence similarity to Pasteuria penetrans （99.3%）and grouped with P. penetrans in the phylogenetic tree. Based on the morphological characteristics and molecular phylogenetic analysis, strain PPh01 was identified as Pasteuria penetrans. Parasitism test results showed that spores of PPh01 had infective ability to juveniles of Meloidogyne incognita, M. javonica and M. arenaria.

关 键 词：穿刺巴斯德芽菌 根结线虫 寄生性 

分 类 号：R378.6[医药卫生—病原生物学]