基质金属蛋白酶活性测定的改良底物胶电泳法  

Modified Gelatin-PAG Electrophoresis for Determination of Activity of Matrix Metalloproteinase

作　　者：丁廷波[1] 杨渝珍[1] 韩玲[1] 王开富 王震 

机构地区：[1]同济医科大学实验医学研究中心分子生物学研究室,武汉430030

出　　处：《同济医科大学学报》2000年第1期43-45,48,共4页Acta Universitatis Medicinae Tongji

基　　金：国家自然科学基金!(No.39630320)

摘　　要：基于ＳＤＳ－聚丙烯酸胺凝胶电泳（ＳＤＳ－ＰＡＧＥ）的原理和技术方法，介绍一种改良的明胶－聚丙烯酰胺凝胶电泳方法（Ｇｃｌａｔｉｎ－ＰＡＧＥ），简称底物胶电泳法，用于测定能够水解明胶的基质金属蛋白酶类（ＭＭＰｓ）的水解活性。该方法简便实用，将底物胶中明胶浓度从 １０ ｇ／Ｌ降至 ２ ｇ／Ｌ，并将分子质量标准浓度提高至≤４０μｇ／孔，较好地解决了实验过程中所遇到的有关分子质量鉴定的难题。On the basis of the principles and techniques of SDS-PAG electrophoresis, a new modified Gelatin-PAGE method, called as substance -gel electrophoresis, was introduced. This modified method can be used to determine the activity of matrix metalloproteinase which had capacity of digesting gelatin. This method decreased the concentration of gelatin mixed with PAG from 10 g/L to 2 g/L, and increased the molecular weight standard concentration to ≤40 μg/well. It is simple and practical.

关 键 词：基质金属蛋白酶 电泳 聚丙烯酰胺凝胶 

分 类 号：Q559[生物学—生物化学] R446.11[医药卫生—诊断学]