超高效液相色谱-二极管距阵检测器法同时测定水中7种有机酚  

Simultaneous determination of seven organic phenols in water by ultra performance liquid chromatography with photodiode array detection

作　　者：陆蓓蓓[1,2] 单晓梅[1,3] 沈登辉[1] 谢继安[3] 张留喜[4] 汤峰[5] 

机构地区：[1]安徽医科大学公共卫生学院,合肥230032 [2]浙江省宁波市疾病预防控制中心,浙江宁波315010 [3]安徽省疾病预防控制中心,合肥230601 [4]安徽省合肥市疾病预防控制中心,合肥230061 [5]合肥供水集团有限公司,合肥230011

出　　处：《中国卫生检验杂志》2012年第6期1214-1216,1220,共4页Chinese Journal of Health Laboratory Technology

基　　金：安徽省自然科学基金资助项目(11040606M216)

摘　　要：目的:建立水中7种有机酚类污染物的超高效液相色谱-二极管距阵检测器测定方法。方法:水样经OasisHLB小柱富集,利用ACQUITY UPLCBEH C8(50 mm×2.1 mm×1.7μm)色谱柱分离,以甲醇和0.1%乙酸溶液为流动相进行梯度淋洗,流速0.25 ml/min,采用PDA检测器,在波长275 nm和303 nm进行测定,外标法定量。结果:7种酚类化合物在0.05 mg/L～5.0 mg/L浓度范围呈线性关系,其相关系数均大于0.999,检出限为15.0μg/L～25.0μg/L,方法回收率在86.9%～104.1%,相对标准差均小于5%。结论:该法灵敏、高效、准确,适用于水中七种酚类污染物的测定。Objective：To establish an analytical method for determination of seven phenolic pollutants in water by ultra performance liquid chromatography（UPLC）with photodiode array detection（PDA）.Methods：The water samples were extracted and condensed with solid-phase extraction by OasisHLB cartridges,and separation was carried out with ACQUITY UPLC BEH C8（50 mm×2.1 mm×1.7 μm）column.The gradient mobile phase consisted of methanol and 0.1% acetic acid aqueous solution at a flow rate of 0.25 ml/min.Detection was performed with a PDA detector,and detection wavelengths were 275 nm and 303 nm.Quantification was calculated by an external standard method.Results：The linear correlation coefficients of 7 chemicals in the range of 0.05 μg/ml～5.0 μg/ml were all over 0.999.The limits of quantification were between 15.0 μg/L～25.0 μg/L,and the recoveries ranged from 86.9% to 104.1%.Relative standard deviations（RSDs） were all less than 5%.Conclusion：This method is sensitive,efficient,accurate and suitable for the determination of the seven phenolic pollutants in water.

关 键 词：超高效液相色谱 二极管距阵检测器 有机酚 水 

分 类 号：O657.72[理学—分析化学]