空肠弯曲菌分离培养基改良CCD琼脂的性能测试研究  

Evaluation of modified CCD agar for isolation of Campylobacter jejuni

作　　者：何天文[1] 陈佐威[1] 苏丽春[1] 蔡芷荷[2] 吴清平[2] 

机构地区：[1]广东环凯微生物科技有限公司,广州510663 [2]广东省微生物研究所,广州510070

出　　处：《中国卫生检验杂志》2012年第6期1313-1315,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:比较空肠弯曲菌(Campylobacter jejuni)在国内外四个厂家的改良CCD琼脂上生长的灵敏度及回收率。方法:用哥伦比亚血平板作为参照培养基,根据ISO/TS11133.2-2003标准检验方法对空肠弯曲菌分离培养基进行性能测试。结果:样品本底测试显示,在四个厂家培养基中测试的样品均没有检出可疑的空肠弯曲菌,但厂家②对杂菌的抑制能力较差;各厂家的的改良CCD琼脂灵敏度均可达到10 CFU以内;环凯、厂家①、厂家②、OXOID四个厂家的改良CCD琼脂的回收率分别为83.4%、46.4%、32.4%、89.4%,均能达到ISO/TS11133.2-2003标准检验方法的要求。结论:环凯和OXOID的改良CCD琼脂分离空肠弯曲菌的效果无明显差异,但均优于国内其他两个厂家(P<0.05)。Objective：To compare the sensitivity and recovery rates of four media（modified CCD agar） for isolation of Campylobacter jejuni.Methods：Using the columbia blood agar as the reference medium,the performance of four different media for isolation of C.jejuni was evaluated according to ISO/TS11133.2-2003 in this study.Results：There were no C.jejuni in the samples;The sensitivity of each modified CCD agar was all within 10 CFU.The recovery rates of modified CCD agar from Huankai,brand No.1,brand No.2 and Oxiod were 83.4%,46.4%,32.4% and 89.4% respectively,all could meet the requirement of ISO/TS11133.2-2003.Conclusion：There were no obvious differences between Huankai and Oxiod modified CCD agar for isolating C.jejuni,and they were better than other two.

关 键 词：空肠弯曲菌 选择性分离 培养基 

分 类 号：R446.5[医药卫生—诊断学]