子宫内膜HOXA10及白血病抑制因子mRNA表达水平与体外受精-胚胎移植妊娠结局的关系  

Relationship between expression of endometrial HOXA10,LIF mRNA and the outcome of IVF/ICSI-ET

作　　者：马彩辉[1] 张清学[2] 李予[2] 王文军[2] 麦美琪[2] 杨冬梓[2] 陈向红[2] 

机构地区：[1]解放军第一七四医院生殖医学中心,福建厦门361000 [2]中山大学附属第二医院生殖医学中心

出　　处：《临床医学》2012年第4期1-4,共4页Clinical Medicine

基　　金：广东省医学科研基金(A200316)

摘　　要：目的探讨子宫内膜HOXA10、白血病抑制因子(LIF)mRNA表达水平与体外受精-胚胎移植(IVF/ICSI-ET)妊娠结局的关系。方法前瞻性收集了行IVF/ICSI-ET者59例,其中21例临床妊娠者作为妊娠组,38例未妊娠者作为非妊娠组,于前1个月经周期的黄体中期取内膜,应用RT-PCR技术检测子宫内膜HOXA10 mRNA和LIF mRNA的表达水平。结果妊娠组与未妊娠组黄体中期子宫内膜LIF mRNA水平分别为1.12±0.32和0.94±0.14,差异有统计学意义(P<0.05);妊娠组与未妊娠组分泌中期子宫内膜HOXA10 mRNA水平分别为0.68±0.12和0.62±0.07,差异有统计学意义(P<0.05)。结论分泌中期子宫内膜HOXA10 mRNA和LIF mRNA表达水平与体外受精-胚胎移植妊娠结局有相关性。Objective To evaluate the relationship between expression of endometrial HOXA-10,LIF mRNA and the outcome of IVF/ICSI-ET.Methods Fifty-nine women underwent IVF/ICSI-ET were collected,21 women who successfully became pregnant were selected as pregnant group,38 women who failed to be pregnant were selected as non-pregnant group.RT-PCR was performed to detect the expression of HOXA10 mRNA and LIF mRNA in endometrial samples.Results LIF mRNA in the endometria was 1.12±0.32 in the pregnant group and 0.94±0.14 in the non-pregnant group.The difference between the two groups was significant（P〈0.05）;HOXA10 mRNA in the endometria was 0.68±0.12 in the pregnant group and 0.62±0.07 in the non-pregnant group.The difference between the two groups was significant（P〈0.05）.Conclusion The expression of HOXA10 mRNA and LIF mRNA in endometrium are correlated with the outcome of IVF/ICSI-ET.

关 键 词：HOXA10 白血病抑制因子 体外受精-胚胎移植 子宫内膜容受性 

分 类 号：R714.8[医药卫生—妇产科学]