NOD鼠来源诱导性多能干细胞系的建立及其鉴定  

Generation and identification of pluripotent stem cells from NOD mouse

作　　者：刘海明[1] 张振[1] 杨力[1] 杨锐[1] 杨晓燕[1] 陈客平[1] 孙嘉[1] 吴礼凤[1] 吴焜[2] 陈宏[1] 黄震[1] 蔡德鸿[1] 张桦[1] 

机构地区：[1]南方医科大学附属珠江医院内分泌科,广州510282 [2]南方医科大学病原生物学系

出　　处：《中华内分泌代谢杂志》2012年第6期472-474,共3页Chinese Journal of Endocrinology and Metabolism

基　　金：广东省自然科学基金面上项目（91510515010000602）;教育部新教师基金资助项目（20094433120007）;广东省科技计划项目（2011B031800086）

摘　　要：利用逆转录病毒将Oct4、Sox2、Klf4三个干细胞基因导入NOD鼠尾成纤维细胞，将其诱导为多能干细胞．通过形态观察、干细胞标志检测及多能性分析等方面对其进行鉴定，结果表明该NOD鼠来源诱导性多能十细胞具有类似胚胎干细胞（embryonic stein cells，ES）的特性，表达Es相关基因及蛋h，具有多向分化潜能。来源于NOD鼠体细胞的诱导性多能干细胞有助于构建研究1型糖尿病的体外疾病模型。Three factors （ Oct4, Sox2, and Klf4） were introduc, ed into tail tip-derived fibroblasts of NOD mouse by retrovirus infection. The induced pluripotent stem cells （iPSCs） generated from this method were analyzed in several aspects, including surface antigens, gene expression, alkaline phosphatase activity, and teratoma formation assays. The NOD mouse iPSCs generated from this study had ES-like characters , expressed ES-specifie surface antigens, and possessed the ability to differentiate into 3-germ layers. Such NOD mouse-iPSCs should be useful in tyre 1 dialectic disease modeling, as well as for cell replacement therapy.

关 键 词：诱导性多能干细胞 NOD小鼠 糖尿病 实验性 疾病模型 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]