阿托伐他汀对人脐静脉内皮细胞Lox-1、TNF-α及ICAM-1表达的影响  

Effect of atorvastatin on expression of LOX-1,TNF-α and ICAM-1 in human umbilical vein endothelial cells

作　　者：姜玉姬[1] 姜华[1] 

机构地区：[1]延边大学附属医院肾内科,吉林延吉133000

出　　处：《重庆医学》2012年第17期1708-1709,1712,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(81160476)

摘　　要：目的研究阿托伐他汀(ATV)对脂多糖(LPS)诱导的人脐静脉内皮细胞血凝素样氧化低密度脂蛋白受体-1(LOX-1)、肿瘤坏死因子-α(TNF-α)及细胞间黏附分子(ICAM-1)表达的影响,探讨ATV防治动脉粥样硬化的机制及其抗炎作用。方法体外培养人脐静脉内皮细胞,用LPS刺激人脐静脉内皮细胞后,加入ATV干预24h,收集细胞,用荧光定量PCR方法测定LOX-1、TNF-α及ICAM-1mRNA的表达;用Western blotting法测定LOX-1、TNF-α及ICAM-1蛋白表达。结果用LPS刺激人脐静脉内皮细胞后,引起LOX-1、TNF-α及ICAM-1的高表达,与空白对照组比较差异有统计学意义(P<0.01),用ATV干预以后显著抑制LOX-1、TNF-α及ICAM-1的表达,与模型组比较差异有统计学意义(P<0.01)。结论 ATV可抑制LOX-1、TNF-α及ICAM-1的表达,这可能是其发挥抗动脉粥样硬化作用及抗炎作用的机制之一。Objective To investigate the influence of atorvastatin on the expression of LOX-1,TNFa and ICAM-1 in human umbilical vein endothelial cells （HUVECs） ,and to study its possible anti-atherosclerotic mechanism. Methods HUVECs were cultured in vitro and stimulated with LPS, then treated with atorvastatin for 24 h, finally measured the expression of LOX-1 ,TNF-a and ICAM-1 mRNA with real-time PCR. Western blotting method was used to analyze the expression of LOX 1, TNF-a and ICAM-1 protein. Results LPS enhanced the expression of LOX-1,TNF-a and ICAM-1, with statistical difference compared with the normal control group（P〈0.01）. Atorvastatin decreased the high expression of LOX-1, TNFu and ICAM-1, showing statistical difference compared with the model group（P〈0.01）which were stimulated by LPS. Conclusion Atorvastatin can block the high expression of LOX-1,TNF-a and ICAM-l,which may be one of the mechanisms to play the anti-artherosclerosis and anti-inflammation role.

关 键 词：ATV 内皮细胞 LOX- TNF-Α ICAM-1 动脉粥样硬化 

分 类 号：R96[医药卫生—药理学]