SLC30A3促进人髓性白血病细胞K562向早期红系分化  

SLC30A3 promotes early erythroid differentiation in human erythroleukemia K-562 cell line

作　　者：董林[1] 范翠青[1] 朱宁[1] 陈梅红[1] 

机构地区：[1]中国医学科学院基础医学研究所 北京协和医学院 基础医学院 生物化学与分子生物学系 医学分子生物学国家重点实验室,北京100005

出　　处：《基础医学与临床》2012年第7期773-777,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(31050008)

摘　　要：目的研究SLC30A3在红系分化中的作用。方法在人髓性白血病K562细胞系中用质粒载体过表达SLC30A3后,检测细胞红系分化指标CD235、ε-、γ-和β-珠蛋白的表达量及细胞增殖速度。流式细胞术检测CD235的表达,荧光实时定量PCR检测珠蛋白mRNA水平,Western blot法检测转录因子GATA-2蛋白水平,MTS法检测细胞增殖速度。结果过表达SLC30A3使K562细胞的红系分化特异标志CD235的表达升高,CD235阳性细胞率由对照组的34.25%±16.89%增加至95.7%±0.14%(P<0.01),ε-、γ-和β-珠蛋白的表达明显升高,红系分化相关转录因子GATA-2的表达降低,细胞增殖速度加快。结论 SLC30A3促进人髓性白血病K562细胞系向早期红系分化。Objective To study the role of SLC30A3 in erythroid differentiation. Methods After SLC30A3 was over-expressed by plasmid vector in human erythroleukemia K-562 cell line, markers of erythroid differentiation in- eluding CD235, ε-、γ-and β-globin, as well as cell proliferation rate were measured. Flow cytometry was used to assess the expression of CD235. Quantitative real-time PCR was used to measure the mRNA level of globins. The protein level of transcription factor GATA-2 was evaluated by Western blot. MTS assay was used to check the cell proliferation rate. Results Over-expression of SLC30A3 up-regulated the expression level of erythroid differentia- tion specific marker CD235. The percentage of CD235 （± ） cells increased to 95.7%±0. 14% as compared to that of the negative control which was 34. 25%±16. 89% （P 〈0. 01 ）. The expression of ε-、γ-and β-globin were also remarkably up-regulated. The expression of erythroid down-regulated. The cell proliferation rate was accelerated SLC30A3 promotes early erythroid differentiation in human differentiation-related transcription factor GATA-2 was as a response to SLC30A3 over-expression. Condusions erythroleukemia K-562 cell line.

关 键 词：SLC30A3 红系分化 K562细胞 

分 类 号：R733.7[医药卫生—肿瘤]