黔东南小香鸡mtDNA D-Loop区遗传多态性分析  

Analysis on genetic diversity of mitochondrial DNA D-Loop region of Xiaoxiang chicken in Qiandongnan state

作　　者：李辉[1] 吴婵[1] 熊光源 申学林 

机构地区：[1]贵州大学高原山地动物遗传育种与繁殖教育部重点实验室,贵州贵阳550025 [2]贵州省凯里市畜牧局,贵州凯里556000

出　　处：《广东农业科学》2012年第11期155-157,共3页Guangdong Agricultural Sciences

基　　金：贵州省科技厅农业攻关项目(黔科合NY字[2010]3040号)

摘　　要：采用PCR产物直接测序方法对73只贵州省黔东南小香鸡线粒体DNA控制区第Ⅰ高变区序列进行了分析。结果表明:在所分析的D-Loop区序列(534 bp)中,A、C、G、T 4种碱基的平均含量分别为26.4%、30.20%、13.10%和30.30%;共检测到31个核苷酸多态位点,其中30个为转换、1个为颠换,没有检测到插入/缺失位点;序列共存在21种单倍型,单倍型多样度为0.900,核苷酸多样度为0.01292,表明小香鸡群体内遗传变异较丰富。小香鸡的所有单倍型都与红色原鸡的3个亚种聚在一支,说明小香鸡属于红色原鸡种;在分支内部又有3个分支,揭示小香鸡在遗传组成上具有3个母系血统来源。In this study, the PCR products of the mitochondrial D-Loop sequences （534 bp in length） of 73 individuals from Xiaoxiang chickens of Qiandongnan state, Guizhou province were sequenced directly. The results revealed that the average percentage of A, C, G and T were 26.4%, 29.7%, 13.10% and 30.30% respectively. 31 mutation sites included 30 transitions and 1 transversions and 21 haplotypes were found totally. Haplotype diversity was 0.900 and nucleotide diversity was 0.01292. Xiang chickens of clade were clustered with H19 and gallus. Phylogenetic analysis showed that all the sequences were clustered in 3 major clades. The results in this study indicated that there were 3 maternal origins to this chicken.

关 键 词：小香鸡 线粒体DNA D-LOOP区 遗传变异 核苷酸多样性 

分 类 号：Q343.35[生物学—遗传学]