Chromosomes and Karyotypes of Three Seagrass Species of Hydrocharitaceae  

水鳖科三种海草的染色体及其核型的研究(英文)

作　　者：杨文杰[1] 都晶洁[1] 黄勃[1] 王仁恩[1] 张钰[1] 

机构地区：[1]海南大学海洋学院,海南海口570228

出　　处：《Agricultural Science & Technology》2012年第6期1175-1178,共4页农业科学与技术（英文版）

基　　金：Supported by the State key Subjecet of Botany at Hainan University (071001);the Malor Scientific Research Protect of Hainan Province, China(20080137)~~

摘　　要：[Objective] This study aimed to investigate the chromosomes and karyotypes of three seagrass species of hydrocharitaceae-Enhalus acoroides, Thalassia hemprichii and Halophila minor collected from Li’an Lagoon, Hainan Province, China. [Method] The root-tips of E. acoroides, T. hemprichii and the axillary buds of H. minor were selected as the materials in this study. The conventional crushing method was used to prepare the slice specimens of chromosomes, and the karyotypes of the three species were analyzed. [Result] The chromosome numbers of E. acoroides, T. hemprichii and H. minor were 2n=18, 18, 28, respectively. The karyotype formulas were K （2n）=18= 12m＋6sm, K（2n）=18=12m＋6sm and K（2n）=28=16m＋8sm＋4st, respectively. According to the standard of Stebbins, the karyotypes of E. acoroides and T. hemprichii were 2A, and that of H. minor was 2B. There was no B chromosome and satellite in these three species. [Conclusion] According to the comparison on the karyotypes of this three species, E. acoroides and T. hemprichii are similar in karyotypes to each other, indicating that there is close, inter-relationship between E. acoroides and T. hemprichii.[目的]对采自海南黎安港的3种水鳖科海草—海菖蒲(Enhalus acoroides)、泰来藻(Thalassia hemprichii)和小喜盐草(Halophila minor)的染色体及其核型进行研究。[方法]采用海菖蒲和泰来藻的根尖及小喜盐草的腋芽为材料,采用常规压片法制备染色体玻片标本,并对其进行核型分析。[结果]海菖蒲和泰来藻的染色体数目为 2n=18,小喜盐草的染色体数目为2n=28,两者均未发现B染色体和随体;海菖蒲、泰来藻和小喜盐草的核型公式分别为K(2n)=18=12m+6sm,K(2n)=18=12m+6sm,K(2n)=28=16m+8sm+4st;按Stebbins的核型分类标准,海菖蒲和泰来藻的染色体属2A类型,小喜盐草为2B型。[结论]通过对3种海草核型进行比较发现,海菖蒲与泰来藻的核型差距很小,而它们与小喜盐草的核型差距较大,说明海菖蒲与泰来藻的亲缘关系更近。

关 键 词：Enhalus acoroides Thalassia hemprichii Halophila minor CHROMOSOME KARYOTYPE 

分 类 号：Q943[生物学—植物学]