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作 者:庞帝琼[1] 李健[1] 张蕴薇[1] 于晓丹 杨富裕[1]
出 处:《Agricultural Science & Technology》2012年第6期1247-1250,1269,共5页农业科学与技术(英文版)
基 金:Supported by National Science and Technology Support Project of China (2009BADA7B04, 2011BAD17B01-02);National 973 Project of China (2012CB215300);National 863 Project of China (2012AA101201);National Pasture Modern Industry Technology System of China (CARS-35)~~
摘 要:[Objective] This study aimed to investigate the mutagenic effect of 60 Co γ-rays irradiation on turf characteristics of Buchloe dactyloides (Nutt.) Engelm. [Method] Buffalo grass were irradiated with 60 Co γ-rays at five different radiation intensities (1 200, 1 400, 1 600, 1 800, 2 000 Gy) to determine the related turf characteristics and analyze the mutagenic effect of 60 Co γ-rays at different radiation intensities on buffalo grass. [Result] Germination rates of buffalo grass irradiated by different radiation intensities of 60 Co γ-rays varied inconsistently, and the root length and bud length were shorter than the control; compared with the control, the height of irradiated seedlings was significantly reduced, and the number of tillers, plant height, leaf length and leaf blade width were smaller than the control; however, the stolon length, stolon diameter and number of stolon nods had no significant difference compared with the control. [Conclusion] This study laid foundation for determining the appropriate radiation intensity of 60 Co γ-rays and selecting useful mutants of buffalo grass.[目的]对60Coγ射线辐照对野牛草坪用性状的诱变效应进行研究。[方法]以经不同辐照强度的60Coγ射线处理后的野牛草[Buchloe dactyloides (Nutt.) Engelm.]为试验材料,对其进行坪用相关性状的测定,分析不同γ射线不同辐照强度(1200、1400、1600、1800、2000Gy)对野牛草当代植株草坪相关性状的诱变效应。[结果]各辐照处理的野牛草发芽率变化趋势不一致,种苗的根长和芽长均小于对照;与对照相比,辐照处理后幼苗的高度显著变小,分蘖数、株高、叶长和叶宽均小于对照;匍匐茎长度、匍匐茎粗和匍匐茎节数与对照差异不显著。[结论]该研究为确定野牛草诱变育种的适宜γ射线辐射剂量及筛选有益突变体奠定基础。
关 键 词:Buffalo grass 60 Co γ-rays Turf characteristics Mutagenic effect
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