沙田柚(2x)×柑橘异源体细胞杂种NS(4x)的三倍体后代遗传分析  

Genetic Analysis of Triploid Progenies from Shatian Pummelo(2x)× Citrus Allotetraploid Somatic Hybrid NS(4x)

作　　者：宋健坤[1] 朱世平[1] 谭美莲[1] 郭文武[1] 邓秀新[1] 

机构地区：[1]华中农业大学园艺林学学院,园艺植物生物学教育部重点实验室,武汉430070

出　　处：《园艺学报》2012年第6期1021-1026,共6页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30921002);国家‘863’计划课题(2011AA100205)

摘　　要：用两对SSR引物TAA1和TAA3对以二倍体沙田柚为母本,体细胞杂种NS(Nova橘柚+Succari甜橙)为父本,通过有性杂交和胚挽救获得的79株三倍体后代群体的带型和分离情况进行了分析。结果发现TAA1引物和TAA3引物在后代群体中分别扩增出5种带型和4种带型,子代带型分别符合4︰1︰1︰5︰1和2︰2︰1︰1的分离比例,与根据孟德尔遗传规律推导的双二倍体的分离比例相吻合,初步表明柑橘异源四倍体体细胞杂种减数分裂行为类似于双二倍体。Abstract： Two pairs of SSR （simple sequence repeat） primers TAA1 and TAA3 were used to detect the band patterns and segregation rations among the 79 triploid progenies derived from the sexual cross between diploid Citrus grandis （L.） Osbeck ＇ Shatian＇ pummelo and somatic hybrid NS [ （ C. reticulata Blanco×C. paradisi Macf. ） ＇Nova＇ tangelo ＋ C. sinensis （L.） Osbeck ＇Succari＇ sweet orange] by embyo rescue technology. Five and four band patterns were generated from the TAA1 and TAA3 primers at the ratio of 4 ： 1 ： 1 ： 5 ： 1 and 2 ： 2 ： 1 ： 1 respectively, as was in good accordance with the segregation ratio of amphidiploid deduced by the law of Mendel. The results preliminarily indicated that the meiosis behavior of citrus allotetraploid somatic hybrid was similar to that of the amphidiploid.

关 键 词：柑橘 异源四倍体体细胞杂种 SSR标记 遗传分析 三倍体后代 双二倍体 

分 类 号：S666[农业科学—果树学]