整合农艺性状和分子标记数据构建新疆野苹果核心种质  

Combining Agronomic Traits and Molecular Marker Data for Constructing Malus sieversii Core Collection

作　　者：刘遵春[1,2] 刘大亮[1] 崔美[1] 李敏[1] 焦其庆[1] 高利平[1] 陈学森[1] 

机构地区：[1]山东农业大学作物生物学国家重点实验室,山东泰安271018 [2]河南科技学院园艺园林学院,河南新乡453003

出　　处：《园艺学报》2012年第6期1045-1054,共10页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30871679);国家‘863’计划重点项目(2006AA100108);山东省农业良种工程项目

摘　　要：以新疆野苹果[Malus sieversii(Ledeb.)Roem.]60份初级核心种质为试材,采用不同遗传距离构建新疆野苹果核心种质,利用数量性状参数均值差异百分率(MD)、方差差异百分率(VD)、极差符合率(CR)、变异系数变化率(VR)和分子标记参数多态位点百分率(p)、平均有效等位基因数(MNe)、平均多态信息含量(MPIC)和平均Shannon’s信息指数(MI)等8个指标评价不同方法构建核心种质的优劣,用选出的合适方法构建新疆野苹果核心种质。研究结果表明:利用混合遗传距离(Dmix)构建的核心种质整合了两类不同数据,优于单独使用农艺性状表型值数据或分子标记数据构建的核心种质,其VD为50%,CR为96.56%,VR为117.98%,p为97.67%,MNe为97.15%,MPIC为101.88%,MI为100.44%;利用15个农艺性状检测表明,所构建的42份新疆野苹果核心种质保留了300份原始种质93%以上的农艺性状,很好地代表了原始种质的遗传多样性。To construct Malus sieversii （Ledeb.）Roem. core collection, based on 15 agrinomic traits data and molecular maker from M. sieversii primary core collection, a serials of subsets were sampled at 50% proportion by different genetic distances, respectively. The genetic variation among collections was compared by evaluating the MD （mean difference percentage）, VD （variance difference percentage）, CR （ coincidence rate of range）, VR （ changeable rate of coefficient of variation） of agrinomic traits and the p （percentage of polymorphic loci）, M~ （average effective number of alleles）, Mt （average Shannon＇s information index） and Melc （average polymorphism information content） of molecular marker information. The results showed that the core collection constructed on the basis of a combinationof data for agrinomic traits and molecular marker data is more representative than that constructed on the basis of data from agrinomic traits or molecular marker data alone. The value of VD, CR, VR, p, MNe, MelC and Miis 50%, 96.56%, 117.98%, 97.67%, 97.15%, 101.88% and 100.44%, respectively. The mixed genetic distance （Dmix）is more preferable to construct core collection. The core collection was evaluated by using 15 agrinomic traits, and the data showed that it retained more than 93% agrinomic traits and could highly represent the genetic diversity of the initial collection of 300 Malus sieversii accessions.

关 键 词：新疆野苹果 核心种质 农艺性状 分子标记 

分 类 号：S661.1[农业科学—果树学]