番茄斑萎病毒TSWV的鉴定及抗病种质的筛选  

Identification of Tomato spotted wilt virus and Screening for Resistant Sources in Tomato

作　　者：邱树亮[1] 王孝宣[1] 杜永臣[1] 高建昌[1] 国艳梅[1] 朱德蔚[1] 胡鸿[1] 李宝聚[1] 石延霞[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《园艺学报》2012年第6期1107-1114,共8页Acta Horticulturae Sinica

基　　金：国家‘973’计划项目(2011CB100605);国家自然科学基金项目(31171963);中央级公益性科研院所基本科研业务费专项(1610032011011);‘十二五’国家科技支撑计划项目(2011BAD12B04);农业部园艺作物生物学与种质创制重点实验室项目

摘　　要：在北京顺义地区番茄栽培温室中随机抽取了15份疑似感染番茄斑萎病毒(TSWV)的植株叶片和果实,利用TSWV试剂盒进行DAS-ELISA检测,结果均为阳性,表明其感染了TSWV。利用来自秘鲁番茄的抗TSWV基因Sw-5的共显性SCAR标记,对442份番茄材料进行分析,筛选出24份含有Sw-5的材料,其中4份为野生番茄,1份为杂交种,16份为高代育种自交系,3份为野生潘那利番茄渐渗系。4份野生番茄综合农艺性状较差,如果实小、产量低、品质和风味差等,不能直接应用于育种;其余20份含Sw-5的材料综合农艺性状较好,可作为番茄抗TSWV的育种材料。Fifteen tomato plants which seemed infected Tomato spotted wilt virus （TSWV） were selected randomly from the tomato cultivation greenhouse of Shunyi District, Beijing. Then the leaves and fruits were obtained from each plant and tested for the presence of TSWV using a DAS-ELISA kit. The results showed positive, indicating that 15 tomato materials had been infected with TSWV. A genetic analysis of 442 tomato materials with co-dominant SCAR markers developed from Sw-5, TSWV resistance gene originated from Solanum peruvianum, was issued and identified 24 resistant materials, which including 4 wild tomatoes, 1 hybrid, 16 breeding inbred lines and 3 S. pennellii introgression lines. Those 4 wild tomatoes are not suitable as breeding materials because of the poor agronomic characters, i.e. small fruits, low production, poor quality and bad flavor, while the other 20 tomato materials can be used for developing cultivars resistant to TSWV due to their better agronomic characters.

关 键 词：番茄 番茄斑萎病毒 Sw-5 抗病育种 

分 类 号：S641.2[农业科学—蔬菜学]